Figure 6—figure supplement 1. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 6—figure supplement 1.

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California Institute of Technology, United States
Figure 6—figure supplement 1.
Download figureOpen in new tabFigure 6—figure supplement 1. Hcrt signaling is required for Hcrt neuron-induced increased locomotor activity.

In this experiment, larvae of all six genotypes were tested in the same behavioral plates. Tg(hcrt:ChR2-EYFP);hcrtr+/+ larvae (blue stripes) showed a significant increase in locomotor activity compared to hcrtr+/+ larvae (blue). A similar increase was observed for Tg(hcrt:ChR2-EYFP);hcrtr+/− larvae (cyan stripes) compared to hcrtr+/− larvae (cyan), although the difference was smaller than for hcrtr+/+ larvae, suggesting a dosage effect. Tg(hcrt:ChR2-EYFP);hcrtr−/− larvae (red stripes) were also more active than hcrtr−/− larvae (red), although the difference was much smaller than for Tg(hcrt:ChR2-EYFP);hcrtr+/+ larvae (blue stripes) compared to hcrtr+/+ larvae (blue). Tg(hcrt:ChR2-EFYP);hcrtr+/+ larvae (blue stripes) were also significantly more active than Tg(hcrt:ChR2-EYFP);hcrtr−/− larvae (red stripes). These results suggest that most, but not all, of the effect of stimulating Hcrt neurons on locomotor activity is due to Hcrt signaling. Data is pooled from 3 experiments. Data is represented as mean ± s.e.m. and is plotted as percentage of hcrtr+/+. n indicates number of larvae. *, p < 0.05; **p < 0.01; ***, p < 0.0001 by Steel–Dwass test and correction for multiple comparisons.

DOI: http://dx.doi.org/10.7554/eLife.07000.019