Figure 7. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 7.

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California Institute of Technology, United States
Figure 7.
Download figureOpen in new tabFigure 7. Optogenetic stimulation of Hcrt neurons activates LC neurons.

(A) Schematic representation of areas stimulated (Hcrt, yellow) and imaged (LC, green). a = anterior, p = posterior. (B, C) Representative images of a LC cell expressing GCaMP6s in a Tg(hcrt:EGFP) (B) or Tg(hcrt:ChR2-EYFP) (C) larva before (B, C) and immediately after (B′, C′) stimulation of Hcrt neuron region. (D) GCaMP6s fluorescence intensity for a representative LC neuron in a Tg(hcrt:EGFP) (top) and a Tg(hcrt:ChR2-EYFP) (bottom) larva. Single-trial (gray) and average (black) responses are shown. (E) Mean ± s.e.m. ∆F/F (%) values averaged for all trials. n indicates number of trials for 5 Tg(hcrt:EGFP) and 4 Tg(hcrt:ChR2-EYFP) larvae. ***, p < 0.001 by one-way ANOVA. See Videos 1 and 2 for examples of GCaMP6s imaging.

DOI: http://dx.doi.org/10.7554/eLife.07000.020