Figure 2—figure supplement 1. | Cell-to-cell infection by HIV contributes over half of virus infection

Open accessCopyright infoDownload PDFDownload figures

Cell-to-cell infection by HIV contributes over half of virus infection

Figure 2—figure supplement 1.

Affiliation details

Kyushu University, Japan; Japan Science and Technology Agency, Japan; Kyoto University, Japan; University of Tokyo, Japan; Hospital Saint Louis, France; Université Paris Diderot, Sorbonne Paris Cité, France; Tokyo University of Agriculture, Japan
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. No effect of the shaking procedure on HIV-1 cell-free infection.

Jurkat cells were infected with HIV-1 (at multiplicity of infection 1) as described in ‘Materials and methods’, and the infected cells were cultured in the static and the shaking condition. By harvesting the cells at 24 and 48 hr postinfection, the cells were analyzed by flow cytometry as described in ‘Materials and methods’. The percentage of the average of p24-positive cells are shown with SD. The assay was performed in triplicate, and the representative result is shown. Note that the ratio of input virus to target cells (multiplicity of infection) of this experiment is 10-fold higher than that of the experiment shown in Figure 2. This is for the clear detection of the infected cells (p24-positive cells) during early time points.

DOI: http://dx.doi.org/10.7554/eLife.08150.006