Figure 2—figure supplement 1. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 2—figure supplement 1.

Affiliation details

Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. Validation of select candidate genes in chd4, p53 and zfp-1 RNAi conditions by WISH.

(A) Representative WISH of candidate genes encompassing the full range of expression patterns in control, chd4 and p53 RNAi animals fixed at 3Fd21 (For nkx-2.2, 3Fd18). Genes expressed in mesenchyme, epidermis and gut are reduced in chd4 and p53 RNAi animals. Genes expressed in mesenchyme and epidermis are also reduced in zfp-1(RNAi) animals (3Fd21) but gut expression is not affected. Scale bar: 200 μm. (B) Plot of log2 ratios of candidate genes from (A) in chd4(RNAi) (top panel) and p53(RNAi) (bottom panel) RNA-seq time course.

DOI: http://dx.doi.org/10.7554/eLife.10501.006