Figure 2—figure supplement 3. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 2—figure supplement 3.

Affiliation details

Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 2—figure supplement 3.
Download figureOpen in new tabFigure 2—figure supplement 3. Expression of pla2 and odc-1 in mesenchyme and epidermis.

(A) Triple FISH of pla2, zpuf-6 and AGAT-1 in the mesenchyme. Top row: single channel of each individual gene. Middle row: Left panel, double FISH of pla2 and zpuf-6. Middle panel, double FISH of pla2 and AGAT-1. Right panel, merge. Yellow arrowheads highlight cells that co-express pla2 and zpuf-6 and are AGAT-1neg. Bottom row: Double FISH of pla2 and zpuf-6 in the epidermis (Ep). White arrowheads highlight co-localization. Scale bars: 50 μm. (B) Triple FISH of odc-1, zpuf-6 and AGAT-1 in the mesenchyme. Top row: single channel of each individual gene. Middle row: Left panel, double FISH of odc-1 and zpuf-6. Middle panel, double FISH of odc-1 and AGAT-1. Right panel, merge. Yellow arrowheads highlight cells that co-express odc-1 and zpuf-6 and are AGAT-1neg. Bottom row: Double FISH of odc-1 and zpuf-6 in the epidermis. White arrowheads highlight co-localization. Scale bars: 50 μm.

DOI: http://dx.doi.org/10.7554/eLife.10501.008