Figure 4—figure supplement 2. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Open accessCopyright infoDownload PDFDownload figures

Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 4—figure supplement 2.

Affiliation details

Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 4—figure supplement 2.
Download figureOpen in new tabFigure 4—figure supplement 2. zpuf-6+ cells are enriched at regenerating blastemas.

Shown are posterior trunk fragments cut from wild-type animals and regenerated for the indicated times: hours or days post-amputation (hpa and dpa, respectively) and subjected to double FISH with zpuf-6 and AGAT-1. Dotted lines demarcate the posterior end of the animal. Maximum intensity projections are shown. Scale bar: 100 μm. Quantification of the fractional area of zpuf-6+ cells in the regenerating blastema compared to non-blastema (old tissue). The overall positive signal of zpuf-6+ cells was measured in a fixed region in the blastema compared to the same area in the non-blastema region. Data represent means from 2 regenerating trunk fragments for each time point; error bars: SD. Student's t test: *, p < 0.05.

DOI: http://dx.doi.org/10.7554/eLife.10501.014