Figure 4—figure supplement 3. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 4—figure supplement 3.

Affiliation details

Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 4—figure supplement 3.
Download figureOpen in new tabFigure 4—figure supplement 3. NB.22.1E+ and laminB+ cells exhibit little overlap with zpuf-6 and display slow cell turnover kinetics after irradiation.

(A) Whole-mount FISH of zpuf-6 and markers of epidermal edge cells (NB.22.1E and laminB). zpuf-6 exhibits little to no overlap with NB.22.1E in edge cells (top row) but overlaps with NB.22.1E+ epidermal cells surrounding the ventral mouth opening (middle row, white arrowheads). zpuf-6 shows very little overlap with laminB+ edge cells (bottom row, white arrowheads). Scale bars: 50 μm. (B) Colorimetric WISH of animals after 6,000 Rad irradiation exposure for markers NB.22.1E and laminB. By 14dpi (days post-irradiation), cells around the anterior have disappeared, suggesting that these cells do not turnover as quickly as zpuf-6+ epidermal cells. For NB.22.1E marker, sub-epithelial mesenchymal cells and those around the ventral mouth opening disappear by 7dpi, suggesting they turn over more rapidly than the cells at animal margin. Ventral views. Scale bars: 200 μm.

DOI: http://dx.doi.org/10.7554/eLife.10501.015