Figure 5—figure supplement 2. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 5—figure supplement 2.

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Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 5—figure supplement 2.
Download figureOpen in new tabFigure 5—figure supplement 2. Egr-5 is expressed in multiple post-mitotic epidermal progeny cells.

(A) Whole-mount FISH of egr-5 with various markers in the epidermal lineage. Top panel: Cells expressing the strongest levels of egr-5 exhibit strong AGAT-1 expression and very weak zpuf-6 expression (red arrowheads). Cells expressing lower levels of egr-5 exhibit strong zpuf-6 expression and little to no detectable AGAT-1 expression (yellow arrowheads). Middle panel: Some cells expressing low levels of egr-5 are prog-1+ (white arrowheads). Bottom panel: egr-5 is very weakly expressed in some epidermal cells, which overlap with zpuf-6+ epidermal cells (not shown). Percentages represent fraction of egr-5+ cells (low but detectable expression was counted) that co-express the candidate gene (∼200-400 cells were quantified). Images are single confocal planes. Ep, epidermis. Scale bars: 10 μm. (B) egr-5 transcripts are enriched in the post-mitotic, differentiated cell population (Xins). Average RPKM values are plotted for various known neoblast and non-neoblast genes from wild-type planarian cells dissociated by FACS (data are deposited under GEO accession number: GSE73027). X1 cells are irradiation-sensitive and comprise of genes that are expressed in stem cells (smedwi-1). Xins cells are irradiation-insensitive and represent a population of various post-mitotic cells. chd4 and p53 are expressed in both stem cells and post-mitotic cells and are therefore present in both X1 and Xins cell population. prog-1 and AGAT-1 are post-mitotic epidermal lineage markers and show enrichment in the Xins population. egr-5 has no detectable expression in stem cells based on FISH (not shown), and consistent with this, few egr-5 transcripts are detected in the X1 population.

DOI: http://dx.doi.org/10.7554/eLife.10501.018