Figure 5—figure supplement 3. | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Figure 5—figure supplement 3.

Affiliation details

Stowers Institute for Medical Research, United States; Howard Hughes Medical Institute, Stowers Institute for Medical Research, United States
Figure 5—figure supplement 3.
Download figureOpen in new tabFigure 5—figure supplement 3. Molecular and ultrastructural analysis of egr-5(RNAi) epidermis.

(A,B) Expression patterns of zpuf-6 and rootletin cilia gene marker at (A) 4Fd12 and (B) 4Fd24 after initial RNAi treatment for control and egr-5(RNAi) animals. Representative images are single confocal planes of ventral epidermis (Ep). Scale bars: 50 μm. (C) Scanning electron micrograph of images (1500X) of control and egr-5(RNAi) animals (4Fd12) of the ventral epidermis (Ep) right above the mouth opening. Although cilia are still present in egr-5(RNAi) animals, there is an absence of epidermal pores (red arrowheads) that may be a result of epithelial stretching. Scale bar: 10 μm.

DOI: http://dx.doi.org/10.7554/eLife.10501.019