Figure 4—figure supplement 3. | The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

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The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

Figure 4—figure supplement 3.

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Cornell University, United States
Figure 4—figure supplement 3.
Download figureOpen in new tabFigure 4—figure supplement 3. GST pulldown analysis of Arf1 and Arl1 interaction with Sec7ΔC. 

Stable interaction of Arf1 and Arl1 with S. cerevisiae Sec7ΔC was assayed by pulldown of Sec7 with a great excess of GST-GTPase bound to GDP (denoted D) or GMPPNP (denoted T*). Following SDS-PAGE, protein was visualized by Coomassie staining. There was no apparent nucleotide-dependent interaction observed between Sec7ΔC and Arf1-GTP or Arl1-GTP in this experiment.

DOI: http://dx.doi.org/10.7554/eLife.12411.016