Figure 4—figure supplement 4. | The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

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The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

Figure 4—figure supplement 4.

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Cornell University, United States
Figure 4—figure supplement 4.
Download figureOpen in new tabFigure 4—figure supplement 4. Liposome pelleting analysis of Arf1 and Arl1 interaction with Sec7ΔC. 

Stable interaction of membrane-bound Arf1 and Arl1 with S. cerevisiae Sec7ΔC (as a potential effector) was assayed in the context of membranes by liposome pelleting as described previously (Paczkowski et al., 2012) following EDTA-mediated loading with the indicated nucleotide. The fraction recovered is calculated relative to a baseline of liposome-free pelleting. There was no apparent interaction observed between Sec7ΔC and membrane-bound Arf1-GTP or Arl1-GTP in this experiment.

DOI: http://dx.doi.org/10.7554/eLife.12411.017