Figure 4—figure supplement 5. | The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

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The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

Figure 4—figure supplement 5.

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Cornell University, United States
Figure 4—figure supplement 5.
Download figureOpen in new tabFigure 4—figure supplement 5. Effect of Arl1 preincubation on Sec7 GEF activity. 

S. cerevisiae Sec7ΔC was assayed for rate of nucleotide exchange of Arf1 in the presence of liposomes and Arl1-GMPPNP as described previously (McDonold and Fromme, 2014).

DOI: http://dx.doi.org/10.7554/eLife.12411.018