Figure 7. | The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

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The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase

Figure 7.

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Cornell University, United States
Figure 7.
Download figureOpen in new tabFigure 7. The DCB/HUS domain can inhibit GEF activity in trans

(A) S. cerevisiae Sec7ΔC and isolated GEF constructs were assayed for rate of nucleotide exchange of myristoylated Arf1 in the presence of liposomes, with 16-fold excess DCB/HUS construct or sixfold additional liposomes added as indicated. (B) Wild-type S. cerevisiae Sec7ΔC was assayed for nucleotide exchange of myristoylated Arf1 in the presence of liposomes and a 12-fold excess of DCB/HUS constructs bearing the indicated mutations. The range of activity of interest is bounded by the WT and mock rates and is left unshaded. (C) A speculative model of DCB/HUS domain and GEF domain cooperation in Arf1 activation.

DOI: http://dx.doi.org/10.7554/eLife.12411.026