• Figure 9.
    Download figureOpen in new tabFigure 9. The temporal series regulates the malignant susceptibility of neural cells born during development.

    (A) Larvae were raised at 18°C and heat-shocked in L1 to induce svp-/- MARCM clones. Controls were kept at 18°C, to prevent prosRNAi expression, leading to the persistence of a single NB per clone in adults. In contrast, if larvae are switched to 29°C from late L3, prosRNAi is expressed leading to large tumors in adults, exclusively composed of dNBs expressing Chinmo, Imp and Lin-28. (B) Schematic recapitulation of the above experiments. Blocking temporal progression in NBs extends the window of malignant susceptibility.

    DOI: http://dx.doi.org/10.7554/eLife.13463.036

  • The following dataset was generated:

    Lanet E, Foppolo S, Parrinello H, Rialle S, Maurange C, Narbonne-Reveau K, Dillard C, 2014,The temporal patterning system governs the malignant susceptibility of neural progeny during Drosophila development, www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE64405, Publicly available at NCBI Gene Expression Omnibus (accession no: GSE64405)

  • Figure 4—source data 1.Differentially expressed genes and enriched GO terms and KEGG pathways between prosRNAi tumors expressing various levels of Chinmo.

    (A) Differentially expressed genes between nab>prosRNAi and nab>prosRNAi, chinmoRNAi late larval VNCs (p-value > 0,05). (B) Differentially expressed genes between nab>prosRNAi, chinmo and nab>prosRNAi late larval VNCs (p-value > 0,05). (C) List of genes that are commonly up- and down-regulated when comparing nab>prosRNAi vs. nab>prosRNAi, chinmoRNAi VNCs and nab>prosRNAi, chinmo and nab>prosRNAi VNCs. (D) Differentially expressed genes between nab>prosRNAi, chinmo and nab>prosRNAi, chinmoRNAi late larval VNCs (p-value > 0,05). (E) Enriched KEGG pathways for commonly up-regulated genes (see C). (F) Enriched GO for up-regaled and down-regulated genes.

    DOI: http://dx.doi.org/10.7554/eLife.13463.018

    Download source data [figure-4—source-data-1.media-1.xls]