• Table 1.

    Results of omnibus statistical tests of experiments for the general characterization of α2CA1KO, α2CA3KO and α2DGKO mice.

    DOI: http://dx.doi.org/10.7554/eLife.14120.009

    1. Immunohistochemistry
    One-Way ANOVA; Factor: Genotype
    α2 Subunitα1 Subunitα5 Subunit
    CA1F(3,10)=9.44P=0.003F(3,9)=0.08p=0.97F(3,9)=0.10p=0.96
    CA3F(3,10)=5.05P=0.02F(3,9)=0.01p=0.99F(3,9)=0.13p=0.94
    DGF(3,10)=9.08P=0.003F(3,9)=0.00p=1.00F(3,9)=0.06p=0.98
    CortexF(3,10)=1.90p=0.19F(3,9)=0.01p=0.83F(3,9)=0.01p=0.99
    AmygdalaF(3,10)=1.28p=0.34F(3,9)=0.21p=0.89F(3,9)=0.36p=0.78
    2. Quantitative PCR
    One-Way ANOVA; Factor: Genotype
    α2 Subunitα3 Subunitα4 Subunit
    CA1F(3,16)=10.66p<0.001F(3,16)=0.10p=0.96F(3,16)=2.48p=0.1
    CA3F(3,16)=10.53p<0.001F(3,16)=0.67p=0.58F(3,16)=4.74p=0.02
    DGF(3,16)=7.32p=0.003F(3,12)=1.96p=0.17F(3,12)=2.90p=0.08
    CortexF(3,16)=15.69p<0.001
    AmygdalaF(3,16)=3.01p=0.06
    3. Slice Electrophysiology
    Two-Way Mixed Factorial ANOVA; Factors: Genotype (between-subjects), Drug (within-subjects)
    CA1AmplitudeFrequencyDecay Time
    GenotypeF(1,38)=1.83p=0.18F(1,38)=2.80p=0.10F(1,38)=1.94p=0.17
    DrugF(1,38)=1.49p=0.23F(1,38)=9.38p=0.004F(1,38)=107.96p<0.001
    Genotype x Drug InteractionF(1,38)=2.91p=0.09F(1,38)=3.11p=0.08F(1,38)=0.82p=0.37
    CA3AmplitudeFrequencyDecay Time
    GenotypeF(1,21)=2.36p=0.14F(1,21)=1.85p=0.19F(1,21)=0.95p=0.34
    DrugF(1,21)=0.66p=0.42F(1,21)=0.09p=0.77F(1,21)=30.54p<0.001
    Genotype x Drug InteractionF(1,21)=0.21p=0.65F(1,21)=1.90p=0.18F(1,21)=0.25p=0.62
    DGAmplitudeFrequencyDecay Time
    GenotypeF(1,21)=1.50p=0.23F(1,21)=1.58p=0.22F(1,21)=0.01p=0.91
    DrugF(1,21)=2.58p=0.12F(1,21)=1.58p=0.22F(1,21)=47.42p<0.001
    Genotype x Drug InteractionF(1,21)=0.54p=0.47F(1,21)=1.33p=0.26F(1,21)=0.30p=0.59
    4. Tests of Hippocampal Function
    Delay – Trace Fear Conditioning
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Condition (between-subjects)
    % Freezing
    GenotypeF(3,49)=0.38p=0.77
    ConditionF(1,49)=41.57p<0.001
    Genotype x Cond. InteractionF(3,49)=0.71p=0.55
    Contextual Fear Conditioning
    One-Way ANOVA; Factor: Genotype (between-subjects)
    % Freezing
    GenotypeF(3,35)=5.47p=0.003
    Morris Water Maze
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Day (within-subjects)
    Time to platform
    GenotypeF(3,80)=5.17p=0.01
    DayF(5,80)=244.12p<0.001
    Genotype x Day InteractionF(5,80)=1.36p=0.19
  • Table 2.

    Results of omnibus statistical tests of measured parameters in behavioral tests of anxiety and general locomotion.

    DOI: http://dx.doi.org/10.7554/eLife.14120.013

    1. Elevated Plus Maze (C57BL/6J)
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
    % Open Arm Time% Open Arm EntriesDistance Travelled
    GenotypeF(3,75)=1.41p=0.25F(3,75)=0.13p=0.94F(3,75)=0.85 F(3, 69) = 1.11p=0.47
    DrugF(1,75)=16.48p<0.001F(3,75)=2.33p=0.13F(3,75)=0.61p=0.44
    Genotype x Drug InteractionF(3,63)=1.54p=0.21F(3,75)=1.47p=0.23F(3,75)=1.13p=0.34
    1’. Elevated Plus Maze (129X1/SvJ)
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
    % Open Arm Time% Open Arm EntriesDistance Travelled
    GenotypeF(3,55)=0.17p=0.92F(3,55)=1.59p=0.20F(3,55)=1.17p=0.33
    DrugF(1,55)=11.49P=0.001F(1,55)=3.4649p=0.07F(1,55)=1.28p=0.29
    Genotype x Drug InteractionF(3,55)=2.28p=0.09F(3,55)=0.50p=0.69F(3,55)=0.82p=0.49
    2. Light / Dark Box
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
    % Time in LightEntries to Light
    GenotypeF(3,73)=5.03p=0.003F(3,73)=0.84p=0.48
    Drug(F(1,73)=26.00p<0.001F(1,73)=1.45p=0.23
    Genotype x Drug InteractionF(3,73)=5.53p=0.002F(3,73)=2.17p=0.09
    3. Open Field
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
    Distance Travelled
    GenotypeF(3,56)=1.78p=0.16
    DrugF(1,56)=0.04p=0.84
    Genotype x Drug InteractionF(3,56)=0.43p=0.73
  • Table 3.

    Results of omnibus statistical tests of measured parameters in behavioral tests of fear.

    DOI: http://dx.doi.org/10.7554/eLife.14120.017

    1. Fear-Potentiated Startle
    Within-Genotype Comparisons
    Two-Way Factorial ANOVA; Factors: Tone/No Tone (within-subjects), Drug (between-subjects)
    α2F/Fα2CA1KO
    ToneF(1,28)=33.75p<0.001F(1,20)=49.17p<0.001
    DrugF(1,28)=0.30p=0.59F(1,20)=0.51p=0.48
    Tone x Drug InteractionF(1,28)=9.95p=0.004F(1,20)=0.02P=0.89
    α2CA3KOα2DGKO
    ToneF(1,18)=16.60p<0.001F(1,20)=54.46p<0.001
    DrugF(1,18)=0.09p=0.77F(1,20)=0.73p=0.40
    Tone x Drug InteractionF(1,18)=5.16p=0.04F(1,20)=10.24p=0.01
    Between-Genotype Comparisons
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
    % FPS
    GenotypeF(3,86)=1.17p=0.91
    DrugF(1,86)=16.69p<0.001
    Genotype x Drug InteractionF(3,86)=2.44p=0.07
    2. Vogel Conflict Test
    Pretest (Unpunished) Drinking
    One-Way ANOVA; Factor: Genotype (between-subjects)
    Number of licks
    GenotypeF(3,63)=0.63p=0.60
    Test (Punished) Drinking
    Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
    Number of licks
    GenotypeF(3,59)=1.00p=0.40
    DrugF(1,59)=14.57p<0.001
    Genotype x Drug InteractionF(3,59)=1.21p=0.31
  • Table 4.

    Omnibus statistical tests of measured parameters in in vivo LFP recordings collected from ventral and dorsal hippocampus.

    DOI: http://dx.doi.org/10.7554/eLife.14120.021

    VENTRAL HIPPOCAMPUS
    1. Peak Theta Frequency
    Two-Way ANOVA; Factors: Stimulation intensity (within-subjects), Time before/after drug (within-subjects)
    α2F/Fα2CA1KO
    Stimulation intensityF(4,32)=9.13p<0.001F(4,24)=18.12p<0.001
    Time after drugF(2,32)=22.98p<0.001F(2,24)=37.01p<0.001
    Stimulation x TimeF(8, 32)=4.27p<0.001F(8,24)=9.07p<0.001
    α2CA3KOα2DGKO
    Stimulation intensityF(4,32)=8.14p<0.001F(4,32)=3.84P=0.02
    Time after drugF(2,32)=6.14P=0.02F(2,32)=2.47P=0.15
    Stimulation x TimeF(8, 32)=3.14P=0.01F(8, 32)=6.63p<0.001
    2. Stimulation Intensity – Theta Frequency Slope
    One-Way ANOVA; Factor: Genotype (between-subjects)
    30min post-diazepam60min post-diazepam
    GenotypeF(3,15)=3.94p=0.03F(3,73)=0.84p=0.48
    3. Normalized Power
    One-Way ANOVA; Factor: Genotype (between-subjects)
    60min post-diazepam
    GenotypeF(3,15)=4.65p=0.02
    DORSAL HIPPOCAMPUS
    4. Peak Theta Frequency
    Two-Way ANOVA; Factors: Stimulation intensity (within-subjects), Time before/after drug (within-subjects)
    α2F/Fα2CA1KO
    Stimulation intensityF(4,32)=8.19p<0.001F(4,32)=5.29p=0.01
    Time after drugF(2,32)=31.65p<0.001F(2,32)=3.03p<0.11
    Stimulation x TimeF(8,32)=8.87p=0.003F(8,32)=1.89p=0.10
    α2CA3KOα2DGKO
    Stimulation intensityF(4,40)=19.19p<0.001F(4,32)=8.86p<0.001
    Time after drugF(2,40)=27.08p<0.001F(2,32)=9.25p=0.01
    Stimulation x TimeF(8,40)=4.30p<0.001F(8,32)=2.39p=0.04
    5. Stimulation Intensity – Theta Frequency Slope
    One-Way ANOVA; Factor: Genotype (between-subjects)
    30min post-diazepam60min post-diazepam
    GenotypeF(3,17)=2.97p=0.14F(3,17)=4.32p=0.02
    6. Normalized Power
    One-Way ANOVA; Factor: Genotype (between-subjects)
    60min post-diazepam
    GenotypeF(3,15)=1.02p=0.41