Figure 1. | Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels

Open accessCopyright infoDownload PDFDownload figures

Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels

Figure 1.

Affiliation details

Harvard University, United States; Howard Hughes Medical Institute, Harvard University, United States
Figure 1.
Download figureOpen in new tabFigure 1. NaV1.7 Optopatch Spiking (NaV1.7-OS) HEK cells.

(A) Genes expressed heterologously in NaV1.7-OS HEK cells. Kir2.1 maintains a hyperpolarized resting potential close to the K+ reversal potential. NaV1.7 imparts electrical excitability. CheRiff depolarizes the cells upon optical excitation and can trigger a NaV1.7-mediated action potential. QuasAr2 is excited by red light and emits near infrared fluorescence in a voltage-dependent manner. (B) Epifluorescence images of QuasAr2 and CheRiff-eGFP expressed in NaV1.7-OS HEK cells. Scale bar 10 μm.

DOI: http://dx.doi.org/10.7554/eLife.15202.003