Figure 2—figure supplement 1. | Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels

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Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels

Figure 2—figure supplement 1.

Affiliation details

Harvard University, United States; Howard Hughes Medical Institute, Harvard University, United States
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. Current clamp recording of light triggered action potentials in Nav1.7-OS HEK cells.

(A) An action potential recorded via manual patch clamp from a Nav1.7-OS HEK cell cluster stimulated by 20 ms blue light pulse at 50 mW/ cm2. The dashed line indicates the firing threshold. (B) Plateau potential induced by different intensities of blue light stimulation. Current clamp recordings were performed on Nav1.7-OS HEK cell clusters stimulated with 500 ms blue light ranging from 1.1 to 84 mW/ cm2. (C) Membrane potential at 400 ms after onset of blue light stimulus as a function of the blue light intensity.

DOI: http://dx.doi.org/10.7554/eLife.15202.005