Figure 5—figure supplement 1. | Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels

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Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels

Figure 5—figure supplement 1.

Affiliation details

Harvard University, United States; Howard Hughes Medical Institute, Harvard University, United States
Figure 5—figure supplement 1.
Download figureOpen in new tabFigure 5—figure supplement 1. Fluorescence traces from NaV1.5-OS HEK cells with different drugs.

NaV1.5-OS HEK cells were stimulated with eight pulses of blue light (20 ms, 50 mW/cm2) at 4 Hz, and QuasAr2 fluorescence was monitored with 635 nm excitation, 400 W/cm2. TTX and PF-04856264 had little effect on channel function. Amitriptyline and trifluoperazine showed strong use-dependent block. Isradipine and iloperidone showed use-dependent block with fast recovery, leading to alternating response amplitudes.

DOI: http://dx.doi.org/10.7554/eLife.15202.014