Figure 1—figure supplement 3. | Human kinetochores are swivel joints that mediate microtubule attachments

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Human kinetochores are swivel joints that mediate microtubule attachments

Figure 1—figure supplement 3.

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University of Warwick, United Kingdom
Figure 1—figure supplement 3.
Download figureOpen in new tabFigure 1—figure supplement 3. The experimental setup to measure intra-kinetochore distance from single time point z-stacks.

Live cell imaging of eGFP-CENP-A and Nnf1-Alexa594 in microscope x’z’ plane demonstrates imaging of both eGFP and Alexa594 across the entire z'-stack. Images in microscope x’y’ plane demonstrate Gaussian-fitted spot centres. Schematic shows measurements of intra- (Δ) and inter- (d) kinetochore distances. Scale bars 500 nm.

DOI: http://dx.doi.org/10.7554/eLife.16159.006