Figure 3—figure supplement 1. | Human kinetochores are swivel joints that mediate microtubule attachments

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Human kinetochores are swivel joints that mediate microtubule attachments

Figure 3—figure supplement 1.

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University of Warwick, United Kingdom
Figure 3—figure supplement 1.
Download figureOpen in new tabFigure 3—figure supplement 1. Measurement of axial swivel, and characterisation of 1D delta’s underestimate of 3D delta.

(A) Schematic of a kinetochore’s inner (CENP-A; green circles) and outer domain (Ndc80; red circles) markers relative to its sister in the xy-plane. Green and black dashed lines represent sister-sister and marker-marker axes, respectively. Measurements shown are the inter-kinetochore distance (dy; black), intra-kinetochore distance (Δy,1; light blue), and a third distance measurement, εy,1(defined as the distance between a kinetochore’s outer domain marker and its sister’s inner domain marker; purple), and kinetochore swivel (ϑy,1; dark blue). (B) The triangle formation used to measure axial swivel of a kinetochore. (C) Schematic of a kinetochore pair, as in (A), however both inner-inter-kinetochore (dy,1; between inner domain markers) and outer-inter-kinetochore (dy,2; between outer domain markers) are shown. (D) The triangle formation used to calculate the underestimate of 3D intra-kinetochore distance using 1D measurements.

DOI: http://dx.doi.org/10.7554/eLife.16159.014