Figure 4—figure supplement 1. | Human kinetochores are swivel joints that mediate microtubule attachments

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Human kinetochores are swivel joints that mediate microtubule attachments

Figure 4—figure supplement 1.

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University of Warwick, United Kingdom
Figure 4—figure supplement 1.
Download figureOpen in new tabFigure 4—figure supplement 1. Inner and outer kinetochore markers are generally co-linear with their attached k-fibre.

(A) Kinetochores exhibiting swivel marked by eGFP-CENP-A, Nnf1-Alexa594 and αTubulin-Alexa647. Green and red crosses represent the centre of eGFP and Alexa594 spots, respectively; dashed blue lines represent kinetochore-microtubule (kMT) axes determined from k-fibre fluorescence close to the kinetochore. Scale bars 200 nm. The schematic demonstrates the measurement of the angle tended by the kMTs on the sister-sister axis, ϑ kMT. (B) Distributions of ϑy-swivel (gold; n = 2427) and ϑ kMT (blue; n = 610) in cells expressing eGFP-CENP-A and Nnf1-Alexa594. (C) 2D histogram of absolute ϑ kMT versus absolute ϑ y-swivel, which are significantly correlated (r = 0.371, p < 10–4, n = 573; see supplementary material), demonstrating that kMTs are generally aligned along the intra-kinetochore axis. (D) Permutation test of significance of correlation between absolute ϑy-swivel and absolute ϑ kMT (blue), and the best fit Gaussian distribution to the data (red; µ = 2.1 × 10–6, σ = 0.042), with Monte Carlo sampling of 106 permutations. Red dashed line is the observed correlation between ϑy-swivel and ϑ kMT, which demonstrates the significance of correlation (p = 2.8 × 10–19, z = 8.9).

DOI: http://dx.doi.org/10.7554/eLife.16159.017