Figure 6—figure supplement 2. | Transient rapamycin treatment can increase lifespan and healthspan in middle-aged mice

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Transient rapamycin treatment can increase lifespan and healthspan in middle-aged mice

Figure 6—figure supplement 2.

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University of Washington, United States; Fred Hutchinson Cancer Research Center, United States; University of Missouri, United States
Figure 6—figure supplement 2.
Download figureOpen in new tabFigure 6—figure supplement 2. Bacterial DNA in fecal samples significantly different between control and rapamycin.

(A). Prevalence distribution of operation taxonomy units (OTUs) (Log2 of 16 rRNA gene abundance) in fecal samples that have p-values less than 0.05 using the R package metagenomeSeq, contrasting control and rapamycin group after controlling for the delivery method, gender and mouse batch effects. CT and RP indicate control and rapamycin, respectively. (B) The ratio of SFB DNA to total bacterial DNA in fecal samples measured by Real-time PCR divided by sex and treatment method. N = 4–10. Data are indicated as mean ± s.e.m. *p<0.05. **p<0.01. (C) Semi-quantitative grading of SFB amount in the intestinal tissue section. 0 indicates the absence. 1–4 indicates the grades of the SFB amount with 1 lowest and 4 highest.

DOI: http://dx.doi.org/10.7554/eLife.16351.026