Figure 2—figure supplement 5. | Presence versus absence of CYP734A50 underlies the style-length dimorphism in primroses

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Presence versus absence of CYP734A50 underlies the style-length dimorphism in primroses

Figure 2—figure supplement 5.

Affiliation details

University of Potsdam, Germany; University of Zurich, Switzerland; RIKEN Center for Sustainable Resource Science, Japan; University of Oxford, United Kingdom; University of Oslo, Norway; Leibniz Institute of Plant Genetics and Crop Plant Research, Germany; Gartenbau Ebbing-Lohaus, Germany
Figure 2—figure supplement 5.
Download figureOpen in new tabFigure 2—figure supplement 5. Characterization of long-homostylous mutants of P. vulgaris and P. x pruhoniciana.

(A) Phenotype of long homostyle mutant of P. x pruhoniciana. Blue arrowheads indicate the position of the stigma, yellow arrowheads indicate the position of anthers. Scale bar is 5 mm. (B) PCR-genotyping of S- and L-morph P. x pruhoniciana individuals, three long homostyle mutant P. x pruhoniciana plants and one long homostyle mutant P. vulgaris individual. Multiplex PCR was performed using primers to amplify the indicated CYP734A50 exons and ITS as an internal control. Arrowheads mark CYP734A50 specific bands; asterisks indicate exon-4 deletion in the mutants. Three different primer pairs were used to test for the presence of exon 4. Each genotyping PCR was performed twice independently. (C) Expression of full-length CYP734A50 transcript and of TUBULIN control in S- and L-morph plants, as well as the long homostyle exon-4 deletion mutant from the commercially grown P. vulgaris population. Fragment lengths of DNA-size standard are indicated. RT: reverse transcription.

DOI: http://dx.doi.org/10.7554/eLife.17956.014