Figure 2—figure supplement 6. | Presence versus absence of CYP734A50 underlies the style-length dimorphism in primroses

Open accessCopyright infoDownload PDFDownload figuresRelated content

Presence versus absence of CYP734A50 underlies the style-length dimorphism in primroses

Figure 2—figure supplement 6.

Affiliation details

University of Potsdam, Germany; University of Zurich, Switzerland; RIKEN Center for Sustainable Resource Science, Japan; University of Oxford, United Kingdom; University of Oslo, Norway; Leibniz Institute of Plant Genetics and Crop Plant Research, Germany; Gartenbau Ebbing-Lohaus, Germany
Figure 2—figure supplement 6.
Download figureOpen in new tabFigure 2—figure supplement 6. Virus-induced gene silencing in P. forbesii.

(A) Phenotypes of P. forbesii seedlings injected with empty TRV-vectors as negative control (left) and with PDS-containing TRV vectors (right) showing bleaching due to silencing of phytoene desaturase (PDS) expression. No phenotypic alteration is seen in the negative control seedlings compared to untreated plants. Scale bar is 1 cm. (B) Flower phenotypes from the indicated treatments. Mock indicates injection of the inflorescence stems with water. (C,D) Anther height (C) and style length (D) of plants from the indicated treatments. Numbers of flowers for stamen and style-length measurements are indicated. Values are mean ± SD. Means of indicated samples were compared using a two-sided t-test assuming unequal variance, followed by Bonferroni correction. ***p<0.001. (E) Expression of CYP734A50 in dissected styles of plants from the indicated treatments and phenotypes as determined by qRT-PCR, normalized to α-TUBULIN. Each sample (#1, #2, etc) is derived from pooling styles of one individual plant. Values are mean ± SD from three technical replicates per sample.

DOI: http://dx.doi.org/10.7554/eLife.17956.015