Figure 2—figure supplement 3. | Population scale mapping of transposable element diversity reveals links to gene regulation and epigenomic variation

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Population scale mapping of transposable element diversity reveals links to gene regulation and epigenomic variation

Figure 2—figure supplement 3.

Affiliation details

The University of Western Australia, Australia; The Australian National University, Australia
Figure 2—figure supplement 3.
Download figureOpen in new tabFigure 2—figure supplement 3. Example PCR validations for two TE variants.

(A) PCR validations for a TE absence variant. Accessions that were predicted to contain a TE absence are marked in bold. Two primer sets were used; forward (F) and reverse (R) or internal (I). Accessions with a TE absence will not produce the FI band and produce a shorter FR band, with the change in size matching the size of the deleted TE. (B) PCR validations for a TE presence variant. Accessions that were predicted to contain a TE presence are marked in bold. One primer set was used, spanning the TE insertion site. A band shift of approximately 200 bp can be seen, corresponding to the size of the inserted TE.

DOI: http://dx.doi.org/10.7554/eLife.20777.015