Figure 1—figure supplement 1. | Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

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Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

Figure 1—figure supplement 1.

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Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, United States
Figure 1—figure supplement 1.
Download figureOpen in new tabFigure 1—figure supplement 1. MEG-3 localizes before PGL-1 and does not require PGL-1 or PGL-3 to assemble granules.

(A) Photomicrographs of live MEG-3::meGFP in zygotes at three different stages: before polarization (pronuclear formation), during polarization (pronuclear migration) and after polarization (mitosis). These and similar images taken from 3 zygotes at 14 time points were used to quantify MEG-3::meGFP fluorescence levels over time as shown in Figure 1B. (B) Photomicrographs of fixed wild-type and meg-3/4 zygotes after polarization immunostained for PGL-3. meg-3/4 zygotes were derived from meg-3(ax3055); meg-4(ax3052) hermaphrodites. PGL-3 staining was done using the KT3 antibody (DSHB). (C) Photomicrographs of fixed zygotes stained with anti-FLAG (top) and K76 anti-PGL-1 (bottom) to show MEG-4 localization at the 1-cell (left) and 4-cell (right) stages. pgl-1/3 zygotes were derived from pgl-3(bn104); meg-4(ax2080FLAG tag) hermaphrodites treated with pgl-1 RNAi.

DOI: http://dx.doi.org/10.7554/eLife.21337.004