Figure 2—figure supplement 1. | Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

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Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

Figure 2—figure supplement 1.

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Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, United States
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. Expression of MEX-5, and MEX-5(ZF-), MEG-3, and MEG-3IDR.

(A) MEX-5 functions redundantly with MEX-6 to localize MEG-3: Photomicrographs of live wild-type and mex-5(RNAi) zygotes co-expressing mCherry:MEX-5 and MEG-3::meGFP. Loss of mCherry fluorescence in the mex-4(RNAi) zygotes was used to confirm efficient loss of mCherry::MEX-5. Numbers indicate number of zygotes exhibiting phenotype shown / total number of zygotes examined. (B) Western blot of embryo lysates co-blotted with α OLLAS and α tubulin (loading control). Expected sizes for protein-epitope fusions are indicated on the right by a < symbol. N2 lysate is a control lysate expressing no tagged proteins, all others are lysates from embryos expressing tagged proteins as indicated. (C) Bar graph showing relative expression of MEG-3 and MEX-5. Western blot in Figure 2—figure supplement 1 was quantified using Image J. MEX-5 is 10X more abundant than MEG-3.

DOI: http://dx.doi.org/10.7554/eLife.21337.006