Figure 4—figure supplement 2. | Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

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Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

Figure 4—figure supplement 2.

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Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, United States
Figure 4—figure supplement 2.
Download figureOpen in new tabFigure 4—figure supplement 2. Phase Separation assay controls.

(A) Same as above except 3’ fluorescein labeled poly-U RNA was included in the buffer. DIC images (left) show the MEG-3 condensates and fluorescence images (488 channel) show the 3’ fluorescein labeled poly-U RNA concentrated in the condensates. (B) Violin plots showing condensate size and number comparing MEG-3 alone and MEG-3 + MEX-5 (Additional data). As in Figure 4C, the height of the plot shows the area of condensates in μm2. The width of the plot correlates to the proportion of condensates of that size. Numbers inside each violin plot are the total number of condensates pooled from three technical replicates for each condition. Data for the MEG-3 alone sample are the same as in Figure 4C and shown here only for reference.

DOI: http://dx.doi.org/10.7554/eLife.21337.011