Figure 5—figure supplement 1. | Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

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Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3

Figure 5—figure supplement 1.

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Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, United States
Figure 5—figure supplement 1.
Download figureOpen in new tabFigure 5—figure supplement 1. MEG-3IDR in vivo.

Western blot of mixed-stage embryo lysates co-blotted with α OLLAS and α tubulin. Expected sizes for protein-epitope fusions are indicated on the right by a < symbol. Dashed line indicates a break in the original gel. Genotype is indicated on the bottom. The MEG-3IDR is present at higher level than MEG-3, likely due to the fact that unlike MEG-3, MEG-3IDR persists longer in somatic cells (see Figure 5 and data not shown). The significance of this difference is not known. n = 3 technical replicates.

DOI: http://dx.doi.org/10.7554/eLife.21337.013