Figure 1—figure supplement 1. | Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

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Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

Figure 1—figure supplement 1.

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Yale University, United States; University of Georgia, United States; University of Connecticut, United States
Figure 1—figure supplement 1.
Download figureOpen in new tabFigure 1—figure supplement 1. Drosophila melanogaster olfactory behavior toward different culture volumes of Saccharomyces cerevisiae and Acetobacter malorum.

The top three experimental groups are controls: Mock (empty tube versus empty tube) recapitulates alternating of test and control arms, as in all experimental groups; apple cider vinegar (ACV [25% in water]) is the positive control and tested against water only; Benzaldehyde (1%) is the negative control and is a 100-fold dilution of benzaldehyde in paraffin oil (PO) tested against paraffin oil only. In all experimental groups, 10 µl of total volume was used; the culture amount is specified, when appropriate, on the right-hand portion of the plot. The remaining volume in the microbial groups is water. Media control (AJM) is always 5 µl of AJM mixed with 5 µl of water. Data points represent the mean +/- SEM combined from three experiments (n = 12 per experimental group). A one-sample t-test assessed the mean deviance from 0. NS p>0.05; *p≤0.05; **p≤0.01; ***p≤0.001; ****p≤0.0001. Based on this analysis, we used a total microbial culture volume of 5 µl throughout the manuscript, unless otherwise noted.

DOI: http://dx.doi.org/10.7554/eLife.18855.007