Figure 6—figure supplement 1. | Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

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Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

Figure 6—figure supplement 1.

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Yale University, United States; University of Georgia, United States; University of Connecticut, United States
Figure 6—figure supplement 1.
Download figureOpen in new tabFigure 6—figure supplement 1. Acetaldehyde metabolic derivatives can complement the co-culture containing A. pomorum adhA, although their physiological concentrations are unknown.

(A) Dose response of acetaldehyde was given to the co-culture containing A. pomorum adhA (along with a constant dose of 3.0% acetic acid). Metabolite additions were added to the culture in the noted percentages, allowed to sit 35 min at room temperature, mixed 1:1 in water, and assessed for Drosophila attractiveness. Mean ± SEM of 1–7 experiments with 12–42 total replicates per group. The mean rank of fly behavior toward all experimental conditions was compared using the Kruskal-Wallis test followed by Dunn’s post-hoc host multiple comparisons testing. Unique letters indicate difference (p<0.05) (B) Role of acetic acid, acetaldehyde, or specified acetaldehyde metabolic derivatives in complementing the co-culture containing A. pomorum adhA. Acetic acid (3.0%) and acetaldehyde (0.75%) were added and allowed to sit at room temperature for 35 min, mixed 1:1 in water, and Drosophila attraction was assayed. 2,3-butanedione (0.15%), 1,1-diethoxyethane (0.01%), and acetoin (0.15%) were added to the culture, mixed 1:1 with water, and Drosophila behavior was assayed. Mean ± SEM of 2–7 experiments with 5–6 replicates per experiment. The mean rank of fly behavior toward all experimental conditions was compared using the Kruskal-Wallis test followed by Dunn’s post-hoc host multiple comparisons testing. Unique letters indicate difference (p<0.05). Sc = S. cerevisiae, Ap = A. pomorum.

DOI: http://dx.doi.org/10.7554/eLife.18855.044