Figure 7—figure supplement 1. | Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

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Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

Figure 7—figure supplement 1.

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Yale University, United States; University of Georgia, United States; University of Connecticut, United States
Figure 7—figure supplement 1.
Download figureOpen in new tabFigure 7—figure supplement 1. Impact of co-culture metabolites on adult survival and yeast populations.

(A) Drosophila survival in the presence of acetic acid (AA), ethanol (EtOH) or the combination of the two in water. Groupings were based on concentrations of metabolites estimated from pre-ethanol catabolism (9.4% ethanol, High EtOH), middle-staged ethanol catabolism (1.4% ethanol, 2.8% acetic acid, Mod. EtOH, Mod. AA) and post ethanol catabolism (3.42% acetic acid, High AA). Data represent mean +/- SEM of 5–6 replicates of 1 representative experiment of 2. Mod. EtOH, Mod. AA was different from High EtOH condition from 108 hr onward and High AA condition from 72 hr onward (assessed by two-way ANOVA comparing time and condition, p≤0.05, Tukey’s correction). Negative Cntrl is water and Positive Cntrl is Shield’s and Sang M3 Insect Medium. (B) Photograph of yeast populations of a co-culture containing S. cerevisiae and A. malorum adhA (left) and S. cerevisiae and A. malorum WT (right) after growing for 72 hr. 50 ul of the culture was spread onto MRS plates containing a cocktail of antibiotics. Source Data Titles.

DOI: http://dx.doi.org/10.7554/eLife.18855.051