Table 2—source data 1. | Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

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Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

Table 2—source data 1.

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Yale University, United States; University of Georgia, United States; University of Connecticut, United States
Table 2—source data 1.Extracted ion chromatograms of differentially emitted or unique metabolites in the co-culture in Table 2.

Extracted ion chromatograms of differentially emitted or unique metabolites in the co-culture according to solid phase microextraction gas chromatography-mass spectrometry (SPME GC-MS). Specific metabolites are displayed above each panel. For each panel, the left-most plot compares the co-culture containing S. cerevisiae and A. malorum to S. cerevisiae grown alone, A. malorum grown alone, or media (AJM [apple juice medium]); the right-most plot compares the co-culture containing S. cerevisiae and A. pomorum wild-type to the co-culture containing S. cerevisiae and A. pomorum adhA, since A. pomorum adhA is required for Drosophila co-culture preference (Figure 5A). The two plots within the same panel contain the same standard. The y-axis for each plot is the ion current for a m/z value that discriminates the metabolite of interest over a specific retention time window. The following m/z values were chosen for each metabolite based on standards or, in the cases of putative and unknown metabolites (I and J) were chosen from the experimental groups: (A) m/z 74.04 (B) m/z 88.08 (C) m/z 73.03 (D) 87.05 (E) 74.02 (F) 104.04 (G) 60.05 (H) 88.05 (I) 101.06 (J) 101.06. Each panel is one representative replicate of 1 experiment (out of 3–5 total replicates in three experiments).

DOI: http://dx.doi.org/10.7554/eLife.18855.030

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