Figure 2. | A long-term epigenetic memory switch controls bacterial virulence bimodality

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A long-term epigenetic memory switch controls bacterial virulence bimodality

Figure 2.

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The Hebrew University of Jerusalem, Israel
Figure 2.
Download figureOpen in new tabFigure 2. Memory and reset of colony-size bimodality.

(A) Scheme of the experimental procedure for measuring the stability of colony morphotypes: Colonies of EPEC grown on LB agar were picked 1000 min after plating, resuspended, re-plated on LB agar, and subjected to ScanLag analysis. (B) Histogram of the fraction of colonies detected at each time point for bacteria taken from a SMALL colony. (C) Histogram of the fraction of colonies detected at each time point for bacteria taken from a BIG colony. (BC) Experiments were repeated in at least four independent biological replicates. (D, E) The same procedure was repeated for four consecutive cycles using bacteria taken from (D) SMALL or (E) BIG colonies, and in each cycle the fraction of BIG and SMALL colonies was determined. Data are presented as the means ± s.d. of five technical replicates. (F) Scheme of the experimental procedure for the ‘reset’ of the bimodality. (G) SMALL or (H) BIG colonies of EPEC were picked 1000 min after plating, resuspended in LB broth and grown overnight to stationary phase at 37°C. Cultures were then plated and subjected to ScanLag analysis.

DOI: http://dx.doi.org/10.7554/eLife.19599.006