Figure 7—figure supplement 1. | A long-term epigenetic memory switch controls bacterial virulence bimodality

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A long-term epigenetic memory switch controls bacterial virulence bimodality

Figure 7—figure supplement 1.

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The Hebrew University of Jerusalem, Israel
Figure 7—figure supplement 1.
Download figureOpen in new tabFigure 7—figure supplement 1. Ler is differentially expressed in BIG and SMALL colony morphotypes.

(A) EPEC containing a chromosomal ler-gfp fusion was grown for 3 hr in DMEM at 37°C and plated on LB agar at 32°C. ScanLag analysis and colony appearance time was monitored and plotted. (B) BIG and (C) SMALL colonies of ler-gfp EPEC were picked separately after 1000 min, suspended in LB, re-plated on LB agar pads (non-activating conditions. i.e. LB agar at 32°C), and time-lapse microscopy was used to measure the ler-gfp expression during 3 hr of growth. GFP, reflecting ler expression, in bacteria that originated from a SMALL morphotype colony is uniformly high but is bimodal in the bacteria that originated from a BIG morphotype colony. Similar results were obtained in at least five different locations and in two independent biological replicates. Scale bar: 15 μm.

DOI: http://dx.doi.org/10.7554/eLife.19599.016