Figure 9—figure supplement 1. | A long-term epigenetic memory switch controls bacterial virulence bimodality

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A long-term epigenetic memory switch controls bacterial virulence bimodality

Figure 9—figure supplement 1.

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The Hebrew University of Jerusalem, Israel
Figure 9—figure supplement 1.
Download figureOpen in new tabFigure 9—figure supplement 1. Bacteria from SMALL colonies have enhanced self-aggregation properties in liquid culture and increased formation of microcolonies on host cells.

(A) BFP-related aggregation observed directly in EPEC cultures. EPEC bacteria from different conditions were diluted in LB to ~107 bacteria/ml and grown under non-activating conditions. Images were acquired with a scanner. The following cultures were used for inoculation: EPEC culture grown in DMEM for 3 hr at 37°C (Activated), bacteria from suspended SMALL, or BIG colonies (SMALL and BIG respectively), EPEC grown in LB to stationary phase at 37°C (Reset). Note the low turbidity reflecting the absence of planktonic bacteria only in the SMALL well. Scale bar: 4 mm. Similar results were obtained in at least two independent biological replicates. (B) Fluorescent time-lapse microscopy of HeLa cells infected with a 1:1 mixture of wild-type EPEC from BIG and SMALL colonies tagged with YFP and mCherry, respectively (this tagging is the opposite of that in Figure 9B). Similar results were obtained in at least five different locations and in two independent biological replicates. Scale bar: 25 μm.

DOI: http://dx.doi.org/10.7554/eLife.19599.020