Figure 1—figure supplement 1. | Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior in C. elegans

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Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior in C. elegans

Figure 1—figure supplement 1.

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Massachusetts Institute of Technology, United States
Figure 1—figure supplement 1.
Download figureOpen in new tabFigure 1—figure supplement 1. daf-7 expression in the ASJ neuron pair is specific to adult males.

(A–B) FISH images of endogenous daf-7 mRNA in adult hermaphrodite (A) and adult male (B). White dotted lines indicate outline of the ASJ neuron(s). Both images are a single slice through the animal where ptrx-1::gfp was visible and in focus in one or both ASJ neurons. Other neurons visible anterior to the ASI neurons are likely the OLQ neurons, and the cells posterior to the ASJ neurons that express daf-7 mRNA are likely the ADE neurons. Scale bar represents 25 µm. Orientation provided in (A) applies to both images. (C) Fluorescence quantification for daf-7-Cy5 probe in the ASJ neurons (as localized by ptrx-1::gfp) in males and hermaphrodites. ***p<0.001 as determined by unpaired t-test with Welch’s correction. Error bars represent SD. (D) pdaf-7::gfp expression pattern in an L4 larval male. Filled triangles indicate the ASI neurons. Scale bar (shown in C) indicates 50 µm. (E–F) Maximum fluorescence values of pdaf-7::gfp in the ASI (E) and ASJ (F) neurons of developing males. **p<0.01, ***p<0.001, as determined by unpaired t-test with Welch’s correction. Error bars represent SD. n.s., not significant.

DOI: http://dx.doi.org/10.7554/eLife.21166.004