Figure 1—figure supplement 2. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 1—figure supplement 2.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 1—figure supplement 2.
Download figureOpen in new tabFigure 1—figure supplement 2. Validation of shRNA screen hits in tumor-derived cell lines characterized by low (H1650, A549, MCF7, and BT-474) and high (PC9, NCI-H23, H1650-M3, MDA-MB-435s and MDA-MB-231) content of CD44+/CD24− cells.

(A) FACS profile of multiple cancer-derived cell lines used in validation of the shRNA screen hits. Cells were stained with antibodies against cell surface markers CD44 and CD24 and analyzed with FACS. (B) The charts represent the percentage of viable cells 5 days after transfection with the indicated siRNA oligonucleotides compared to scramble siRNA=transfected control cells. Each bar represents mean ± SD of two independent experiments each conducted in eight- replicates (n = 16). (C) Efficiency of knockdown upon transfection with the indicated siRNA oligonucleotides and compared to scramble siRNA transfected control cells. Cells were collected 3 days post transfection and analyzed for expression of the indicated mRNA by RT-qPCR. Each bar represents mean ± SD of three replicates. p-value *<0.05, **<0.005, ***<0.0005, unpaired t-test. Non-significant differences are shown by the symbol ‘#’ or ‘ns’.

DOI: http://dx.doi.org/10.7554/eLife.21615.005