Figure 1—figure supplement 4. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 1—figure supplement 4.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 1—figure supplement 4.
Download figureOpen in new tabFigure 1—figure supplement 4. Validation of shRNA screen hits in H1650-derived isogenic clones, FACS sorted on the basis of surface expression of CD44 and CD24.

(A) FACS profile of H1650-derived isogenic cell lines stained with CD44 and CD24 antibodies. The numbers in bold represent the percentage of cells that are CD44+/CD24−. (B) The charts represent the percentage of viable CD44+/ CD24− cells and cells of other immune types upon transfection with the indicated siRNA oligonucleotides relative to control (scramble siRNA). Each bar represents mean ± SD of three technical replicates from two independent experiments. 10,000 cells were analyzed by FACS for each replicate of each sample. (C) Efficiency of knockdown upon transfection with the indicated siRNA oligonucleotides and compared to scramble siRNA transfected control cells. Cells were collected 3 days post transfection and analyzed for expression of the indicated mRNA. Each bar represents mean ± SD of three replicates. p-value *<0.05, **<0.005, ***<0.0005, unpaired t-test. Non-significant differences are shown by the symbol ‘#’ or ‘ns’.

DOI: http://dx.doi.org/10.7554/eLife.21615.007