Figure 1—figure supplement 6. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 1—figure supplement 6.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 1—figure supplement 6.
Download figureOpen in new tabFigure 1—figure supplement 6. Validation of shRNA screen hits in H1650 and H1650-M3 cells using the clonogenic assay technique.

(A) Clonogenic assay performed on H1650 and H1650-M3 to validate siRNA screen hits for BRCA1, ORC5L, RFC3, and POLS. The charts represent the percentage of viable cells 15 days after transfection with the indicated siRNA oligonucleotides compared to that of scramble-siRNA-transfected control cells. Each bar represents mean ± SD of data collected from ten fields from three different six-well plates (n = 30). (B) Knockdown efficiency for clonogenic assay upon transfection with the indicated siRNA oligonucleotides. Samples were collected 3 days post-transfection and analyzed by immunoblot analysis with antibodies against Total caspase 3, alpha-tubulin and Cleaved-caspase 3. Each bar represents mean ± SD of three replicates. p-value *<0.05, **<0.005, ***<0.0005, unpaired t-test. Non-significant differences are shown by the symbol ‘#’ or ‘ns’.

DOI: http://dx.doi.org/10.7554/eLife.21615.009