Figure 2—figure supplement 1. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 2—figure supplement 1.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. mRNA expression analysis of the shRNA screen hits in tumor-derived cell lines and cells that have been FACS sorted on the basis of their surface expression of CD44 and CD24.

(A) mRNA expression analysis (SYBR-green-based RT-qPCR) of BRCA1, ORC5L, RFC3, RPA2, POLS and ERCC8 in tumor-derived cell lines. The data represent the mean ± SD of three replicates from two independent experiments. (B) mRNA expression analysis (SYBR-green-based RT-qPCR) of BRCA1, ORC5L, RFC3, RPA2, POLS and ERCC8 in H1650 and A549 that have been FACS sorted on the basis of their surface expression of CD44 and CD24. Each bar represents the mean ± SD of three replicates from two independent experiments and represents the mRNA expression of the indicated gene normalized to GAPDH expression of each cell line. p-value **<0.005, unpaired t-test; ns= non-significant.

DOI: http://dx.doi.org/10.7554/eLife.21615.011