Figure 2—figure supplement 3. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 2—figure supplement 3.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 2—figure supplement 3.
Download figureOpen in new tabFigure 2—figure supplement 3. mRNA expression analysis of HDR genes in CD44−/CD24+ and CD44+/CD24− cells FACS sorted from cells lines and patient tumors.

(A) Comparative expression of the indicated HDR genes in FACS-sorted CD44+/ CD24− cells relative to CD44−/ CD24+ cells from H1650, A549 and MCF7. mRNA expression was quantified by RT-qPCR. Each bar is the mean ± SD of three replicates from two different experiments and represents mRNA expression of the indicated gene. p-value *<0.05, **<0.005, unpaired t-test. (B) Schematic of cell sorting from tumors. Tumor-derived single cell suspension was stained with antibodies against CD45, CD31, EpCAM, CD44, and CD24. CD45-; CD31-; EpCAM+ cells were then FACS sorted according to the immune types CD44+/CD24− and CD44−/CD24+. (C) Expression of BLM, BRCA2, NBN and B-ACT genes in FACS sorted CD44−/CD24+ and CD44+/CD24− cells from four NSCL tumors. B-ACT was used as a housekeeping control gene. Each bar represents the mean ± SD of three replicates. p-value *<0.05, ***<0.0005, unpaired t-test.

DOI: http://dx.doi.org/10.7554/eLife.21615.013