Figure 3—figure supplement 1. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 3—figure supplement 1.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 3—figure supplement 1.
Download figureOpen in new tabFigure 3—figure supplement 1. TGF-β signaling is upregulated in FACS-sorted CD44+/CD24− cells.

Expression of well-known TGF-β target genes in CD44+/ CD24− cells relative to CD44−/ CD24+ cells sorted from H1650 and MCF7. mRNA expression was quantified by SYBR-green-based RT-qPCR. Each bar represents the mean ± SD of three replicates from two independent experiments and represents mRNA expression of the indicated gene normalized to GAPDH expression of each cell line (n = 6). p-value *<0.05, **<0.005 unpaired t-test.

DOI: http://dx.doi.org/10.7554/eLife.21615.017