Figure 3—figure supplement 2. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Open accessCopyright infoDownload PDFDownload figuresRelated content

TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 3—figure supplement 2.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 3—figure supplement 2.
Download figureOpen in new tabFigure 3—figure supplement 2. Active TGF-β signaling control the expression of multiple HDR genes.

(A) TGF-β signature target genes measured by SYBR-green-based RT-qPCR in H1650 and A549 upon treating the cells with TGF-β for 9 hr. mRNA expression was quantified by RT-qPCR. Each bar represents the mean ± SD of three replicates from three independent experiments and represents mRNA expression of the indicated gene. p-value *<0.05, **<0.005 , ***<0.0005, paired t-test. (B) mRNA expression analysis of indicated HDR genes in TGF-β-treated cells relative to vehicle control in the indicated cell lines. mRNA expression was quantified by SYBR-green-based RT-qPCR. Each bar represents the mean ± SD of three replicates from two independent experiments (n = 6), normalized to respective GAPDH mRNA expression. Cells were treated for 9 hr with TGF-β (1 ng/ml each of TGF-β1 and TGF-β2). p-value *<0.05, **<0.005 ***<0.0005, paired t-test.

DOI: http://dx.doi.org/10.7554/eLife.21615.018