Figure 4—figure supplement 1. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

Figure 4—figure supplement 1.

Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
Figure 4—figure supplement 1.
Download figureOpen in new tabFigure 4—figure supplement 1. TGF-β treatment increases the number of 53BP1/γ-H2AX double-positive foci in MCF7 cells.

(A) MCF7 cells treated with TGF-β (1 ng/ml each of TGF-β1 and TGF-β2) or DMSO for 3 days and 4 days were stained with antibodies against γ-H2AX (red) and 53BP1 (green). DAPI (blue) was used as a counter-stain. Insets in the left upper corner show a representative nucleus. (B) The chart represents quantification of the experiment depicted in (A). Each bar represents the mean ± SD of the percentage of cells with more than threeγ-H2AX and 53BP1 double-positive foci per field in vehicle or TGF-β-treated cells. Approximately ten fields were counted, for a total of 100 cells (n = 100). (p-value *<0.005, paired t-test).

DOI: http://dx.doi.org/10.7554/eLife.21615.023