Author response image 1. | TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24− cancer cells

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Affiliation details

Cold Spring Harbor Laboratory, United States; Stony Brook University, United States; Huntington Hospital, Northwell Health, United States; University of Southern California, United States; Cancer Research UK – Cambridge Institute, University of Cambridge, United Kingdom
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Download figureOpen in new tabAuthor response image 1. mRNA expression analysis of SMAD3, SERPINE1, SKIL and the indicated HDR genes in vehicle or TGF-β-treated cells relative to vehicle control upon siRNA mediated SMAD3 knock down.

si Scramble was used as an experimental control. mRNA expression was quantified by SYBR-green-based RT-qPCR. Each bar represents mean ± SD of 3 replicates from 2 independent experiments. (p-value * < 0.05, **< 0.005 paired t-test).

DOI: http://dx.doi.org/10.7554/eLife.21615.034