Figure 2. | Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

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Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation

Figure 2.

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University of Glasgow, United Kingdom; Cancer Research United Kingdom Beatson Institute, United Kingdom; University of Vermont, United States
Figure 2.
Download figureOpen in new tabFigure 2. Material transport in between individual parasites within a PV.

(A) FRAP treatment in a conditional act1 cKO 72 hr after excision of act1 (Left panel). FRAP treatment shows no recovery in fluorescence intensity in the bleached cell (arrow) over the duration of the experiment (90 s), indicating that the absence of actin abrogates transport of constitutively expressed YFP between neighbouring cells. FRAP treatment in a wt strain constitutively expressing GFP shows recovery in a bleached cell as soon as 20 s after bleaching (right panel, control experiment). Intensity in the FRAP area was expressed as intensity percentage of the same area in a cell unbleached. Scale bar; 5 µm. See also Videos 1 and 2. FRAP experiments shown are representative for several biological replicates (n > 3). (B) Extracellular vesicles are actively transported in wt parasites. Vesicles positive for GAPM1a-YFP were seen to be transported. Individual vesicles were tracked and their path color coded by frame according to indicated scale. Time is indicated in minutes. Scale bar: 5 µm, detail 1 µm.

DOI: http://dx.doi.org/10.7554/eLife.24119.005