A4,GCA_001593145.1,,,,,,
name,primer,seq,Hit 1 contig,start:end,hit 2contig,hit 2 start:end,
Kary001,HD2/MAT-locu fw,ATATTGGCTCACGTTTCACTGAC,5308,1467:1485,511,8881:8903,
Kary002,HD2/MAT-locus rv,GCCGCTGTCGATTTATGAG,5308,1885:1867,511,9319:9304,
Kary003,HD2/MAT-locus seq,AACCAAGCAAATTCTTGAAGC,5308,1492:1510,511,8901:8926,
,,,,,,,
,,So for A4 it amplifies either:,,,And region with the two opposite genes are,,
,,scaffold5308:1467..1885,,,scaffold5308:1:2739,1:2621,
,,scaffold511:8881..9319,,,scaffold511:718:10167,7185:10093,
,,,,,,,
,,,,,,,
A5,GCA_001593205.1,,,,,,
name,primer,seq,Hit 1 contig,start:end,hit 2contig,hit 2 start:end,
Kary001,HD2/MAT-locu fw,ATATTGGCTCACGTTTCACTGAC,4735,1611:1633,,,
Kary002,HD2/MAT-locus rv,GCCGCTGTCGATTTATGAG,4735,2052:2034,,,
Kary003,HD2/MAT-locus seq,AACCAAGCAAATTCTTGAAGC,4735,1640:1656,,,
,,"Note that there was no clear second contig for the primer BLAST searches, so I blasted the whole region, and got a singel hit to 2225",,,,,
,,So for A5 it amplifies either:,,,And region with the two opposite genes are,,
,,scaffold4735:1611..2052,,,scaffold4735:1:2739,,61:2662
,,scaffold2225:12349..12802,,,scaffold2225:11810:14510,,11796:14533
,,,,,,,
SL1,GCA_003833045.1,,,,,,
name,primer,seq,Hit 1 contig,start:end,hit 2contig,hit 2 start:end,
Kary001,HD2/MAT-locu fw,ATATTGGCTCACGTTTCACTGAC,11992,1185:1207,11897,1503:1525,
Kary002,HD2/MAT-locus rv,GCCGCTGTCGATTTATGAG,11992,778:796,11897,1926:1941,
Kary003,HD2/MAT-locus seq,AACCAAGCAAATTCTTGAAGC,11992,1162:1182,11897,1532:1548,
,,,,,,,
,,So for SL1 it amplifies either:,,,And region with the two opposite genes are,,
,,scaffold11994:778..1207,,,scaffold11994:1..2690,245:2585,
,,scaffold11899:1503..1941,,,scaffold 11899:1..2719,1:2495,