1. Developmental Biology
  2. Evolutionary Biology

Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa

  1. Miguel Salinas-Saavedra  Is a corresponding author
  2. Mark Q Martindale  Is a corresponding author
  1. The Whitney Laboratory for Marine Bioscience, and the Department of Biology, University of Florida, United States
https://doi.org/10.7554/eLife.54927
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  1. Miguel Salinas-Saavedra
  2. Mark Q Martindale
(2020)
Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa
eLife 9:e54927.
https://doi.org/10.7554/eLife.54927
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Abstract

In bilaterians and cnidarians, epithelial cell-polarity is regulated by the interactions between Par proteins, Wnt/PCP signaling pathway, and cell-cell adhesion. Par proteins are highly conserved across Metazoa, including ctenophores. But strikingly, ctenophore genomes lack components of the Wnt/PCP pathway and cell-cell adhesion complexes raising the question if ctenophore cells are polarized by mechanisms involving Par proteins. Here, by using immunohistochemistry and live-cell imaging of specific mRNAs, we describe for the first time the subcellular localization of selected Par proteins in blastomeres and epithelial cells during the embryogenesis of the ctenophore Mnemiopsis leidyi. We show that these proteins distribute differently compared to what has been described for other animals, even though they segregate in a host-specific fashion when expressed in cnidarian embryos. This differential localization might be related to the emergence of different junctional complexes during metazoan evolution.

Introduction

In bilaterians and cnidarians, a polarized epithelium is classically defined as a group of polarized cells joined by belt-like cell-cell junctions and supported by a basement membrane (Magie and Martindale, 2008; St Johnston and Sanson, 2011; Thompson, 2013; Ohno et al., 2015; Salinas-Saavedra et al., 2015). While the asymmetric cortical distribution of the Wnt Planar Cell Polarity (PCP) pathway components polarizes the cells along the tissue plane, the asymmetric cortical distribution of Par system components polarizes the cells along the apical-basal axis (St Johnston and Sanson, 2011; Thompson, 2013; Gumbiner and Kim, 2014; Besson et al., 2015; Yang and Mlodzik, 2015; Ahmed and Macara, 2016; Aigouy and Le Bivic, 2016; Butler and Wallingford, 2017; Davey and Moens, 2017; Salinas-Saavedra et al., 2015; Fanto and McNeill, 2004; St Johnston and Ahringer, 2010; Cha et al., 2011; Kumburegama et al., 2011; Nance and Zallen, 2011; Momose et al., 2012; Wallingford, 2012). The mechanisms that organize cell-polarity are highly conserved in all animals that have been studied and most likely been present in the most recent common ancestor (MRCA) of Cnidaria and Bilateria (Thompson, 2013; Salinas-Saavedra et al., 2015; Kumburegama et al., 2011; Momose et al., 2012; Fahey and Degnan, 2010; Ragkousi et al., 2017; Salinas-Saavedra et al., 2018; Belahbib et al., 2018; Figure 1A).

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Evolution of cell polarity components during animal evolution.

(A) Three major evolutionary steps (left side) that might have changed the organization of cell polarity in the Metazoa. The diagram (right side) depicts the subcellular asymmetric localization of Par proteins in Cnidaria and Bilateria. However, there are no previous descriptions available for ctenophore cells. (B) The stereotyped early development of M. leidyi.

Interestingly, ctenophores or comb jellies, whose position at the base of metazoan tree is still under debate (Dunn et al., 2008; Hejnol et al., 2009; Ryan et al., 2013; Moroz et al., 2014; Whelan et al., 2017), (Simion et al., 2017), (Feuda et al., 2017), possess a stereotyped development (Figure 1B) and do not have the genes that encode the components of the Wnt/PCP pathway in their genomes (Ryan et al., 2013). Thus, the study of the subcellular organization of the Par system components in ctenophores is important to understand the evolution of tissue organization in Metazoa.

The asymmetric localization of the Crumbs (Crb) complex, (e.g. Crb/Pals1/Patj), the Par/aPKC complex (e.g. Par-3/aPKC/Par-6), and the Scribble complex (e.g. Scribble/Lgl/Dlg) in the cortex of bilaterian and cnidarian cells maintains epithelial integrity by stabilizing cell-cell junctions (Ohno et al., 2015; Salinas-Saavedra et al., 2015; Fahey and Degnan, 2010; Salinas-Saavedra et al., 2018; Belahbib et al., 2018) via the Cadherin-Catenin complex (CCC) of mature Adherens Junctions (AJs) (Magie and Martindale, 2008; Belahbib et al., 2018; Harris and Peifer, 2004; Nelson and Nusse, 2004; McGill et al., 2009; Oda and Takeichi, 2011; Schäfer et al., 2014; Weng and Wieschaus, 2016). The maturation of AJs is essential for the maintenance of the Par/aPKC complex localization at the apical cortex that displaces members of the Scribble complex and Par-1 to basolateral localizations associated with Septate Junctions (SJs) (Belahbib et al., 2018; Benton and St Johnston, 2003; Hurov et al., 2004; Zhang et al., 2007; Iden and Collard, 2008; Yamanaka and Ohno, 2008; Oshima and Fehon, 2011; Ganot et al., 2015; Humbert et al., 2015; Kharfallah et al., 2017).

This mechanism is deployed in bilaterian cells to establish embryonic and epithelial cell polarity during early development and is critical for axial organization (Salinas-Saavedra et al., 2015; Cha et al., 2011; Munro, 2006; Patalano et al., 2006; Goldstein and Macara, 2007; Weisblat, 2007; Alford et al., 2009; Munro and Bowerman, 2009; Doerflinger et al., 2010; Chan and Nance, 2013; Lang and Munro, 2017; Tepass, 2012; Nance and Zallen, 2011; Weng and Wieschaus, 2017; Zhu et al., 2017; Ragkousi et al., 2017; Salinas-Saavedra et al., 2018; Schneider and Bowerman, 2003; Macara, 2004; Vinot et al., 2004; Dollar et al., 2005; Ossipova et al., 2005). Components of the Par system are unique to, and highly conserved, across Metazoa, including placozoans, poriferans, and ctenophores (Fahey and Degnan, 2010; Belahbib et al., 2018). But strikingly, ctenophore genomes do not have many of the crucial regulators present in other metazoan genomes (Belahbib et al., 2018; Ganot et al., 2015). For example, none of the components of the Crb complex, a Scribble homolog, or Human and Drosophila SJs, are present (Belahbib et al., 2018; Ganot et al., 2015), and the cytoplasmic domain of cadherin lacks the crucial biding sites to catenins that interact with the actin cytoskeleton (Belahbib et al., 2018). These data raise the question of whether or not ctenophore cells are polarized by mechanisms involving the apicobasal cell polarity mediated by Par proteins. Here, by using antibodies raised to specific ctenophore proteins and confirmed by live-cell imaging of injected fluorescently labeled mRNAs, we describe for the first time the subcellular localization of selected components of the Par system during the development of the ctenophore Mnemiopsis leidyi. Data obtained here challenge the conservation of the apicobasal cell polarity module and raise questions about the epithelial tissue organization as an evolutionary trait of all metazoans.

Results

MlPar-6 gets localized to the apical cortex of cells during early M. leidyi development

We characterized the subcellular localization of the MlPar-6 protein during early M. leidyi development by using our specific MlPar-6 antibody (Figure 2 and Figure 2—figure supplements 1–6). Although MlPar-6 immunoreactivity can be detected in the periphery of the entire cell, in all of over 100 specimens examined, its expression appears to be polarized to the animal cortex (determined by the position of the zygotic nucleus; Figure 2A and Figure 2—figure supplements 8–10) of the single cell zygote and to the apical (animal) cell cortex during every cleavage stage (Figure 2 and Figure 2—figure supplement 3). At the cortex, MlPar-6 localizes to cell-contact-free regions facing the external media (Figure 2C). Gradually through the next three hours of development, MlPar-6 becomes localized to the position of cell-cell contacts by 60 cell stage onwards (Figure 2—figure supplements 3E–G and 4). During gastrulation (3–7 hpf; Figure 2D and Figure 2—figure supplements 3–4), MlPar-6 is not localized in cells undergoing cellular movements including the oral (four hpf; Figure 2—figure supplement 3G) and aboral ectoderm (5–6 hpf; Figure 2D) undergoing epibolic movements, syncytial endoderm, and mesenchymal ‘mesoderm’ (quotation marks its debatable homology). However, this protein remains polarized in ‘static’ ectodermal cells remaining at the animal pole (blastopore) and vegetal pole (4–7 hpf; Figure 2—figure supplements 3F–J and 4). By the end of gastrulation (8–9 hpf; Figure 2E), MlPar-6 becomes localized asymmetrically to the apical cortex of the ectodermal epidermal cells and the future ectodermal pharyngeal cells that start folding inside the blastopore (Figure 2E and Figure 2—figure supplement 5A–C). Interestingly, we do not observe a clear cortical localization in later cydippid stages, and the antibody signal is weaker after 10 hpf in juveniles (Figure 2F). Contrary to expectations, at these later stages, MlPar-6 is cytosolic and does not localize in the cortex of epidermal cells, and a few epithelial and mesenchymal cells showed nuclear localization (Figure 2F). Thereafter, MlPar-6 remains cytosolic in all scored stages up to 24 hpf (Figure 2—figure supplement 6). Cytosolic and nuclear localization of Par-6 has been reported in other organisms when the polarizing roles of this protein are inactive (Mizuno et al., 2003; Johansson et al., 2000; Cline and Nelson, 2007). Thus, our data suggest that MlPar-6 does not play a role in cell polarity during juvenile cydippid stages. These patterns of apical localization seem not to be affected by the cell cycle (Figure 2—figure supplements 8–11). Further work is required to assess the relationship between cell cycle and the localization of these proteins.

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MlPar-6 protein subcellular localization during the early development of M. leidyi.

Immunostaining against MlPar-6 protein shows that this protein localizes asymmetrically in the cell cortex of the eggs (A) and in the cell-contact-free regions of cleavage stages (B–C; white arrows). White circle in C indicates the lack of signal in the cell-contact region. Yellow arrowhead indicates the zygotic nucleus in A. a’ is a magnification of the section depicted in (B) the first cleavage. (D–F): b’ to i’ correspond to magnifications of the regions depicted for each stage. (D) 5–6 hpf, MlPar-6 protein localizes to the apical cortex of the ectodermal cells (Ecto) but is absent from endodermal (Endo) and ‘mesodermal’ (‘Meso’) cells. White arrowhead indicates MlPar-6 protein in regions of cell-contact. Yellow arrowheads indicate the absence of cortical localization. (E) Until 9 hpf, MlPar-6 protein localizes to the apical cortex of the ectoderm (white arrows) and pharynx (white arrowhead) but it is not cortically localized after 10 hpf (F; Yellow arrowheads indicate nuclear localization). Images are maximum projections from a z-stack confocal series. The 8 cell stage corresponds to a single optical section. Orientation axes are depicted in the Figure: Animal/oral pole is to the top. Morphology is shown by DAPI and Tubulin immunostainings. See Figure 2—figure supplements 1–11 for expanded developmental stages. Scale bars: 20 µm.

Similar results were obtained when we overexpressed the mRNA encoding for MlPar-6 fused to mVenus (MlPar-6-mVenus) and recorded the in vivo localization of the protein in M. leidyi embryos (Figure 2—figure supplement 5D–H). Translated MlPar-6-mVenus was observed approximately 4 hr post injection into the uncleaved egg so localization during early cleavage stags was not possible. However, during gastrulation, MlPar-6-mVenus localizes to the apical cell cortex and displays enrichment at the level of cell-cell contacts (Figure 2—figure supplement 5D–F). As we observed by antibody staining, this cortical localization is no longer observable during the cell movements associated with gastrulation and MlPar-6-mVenus remains cytosolic (Figure 2—figure supplement 5D bottom). After eight hpf, MlPar-6-mVenus localizes to the apical cortex of ectodermal epidermal and pharyngeal cells but is not observable in any other internal tissue (Figure 2—figure supplement 5G). After 10 hpf, MlPar-6-mVenus remains in the cytosol and no cortical localization was detectable (Figure 2—figure supplement 5H). Microinjection and mRNA expression in ctenophores is really challenging. For the first time, we have overexpressed fluorescent-tagged proteins for in vivo imaging. In spite of the low number of replicates (see Materials and methods), our results are consistent with the antibody observations presented above.

MlPar-1 remains cytoplasmic during early M. leidyi development

In bilaterians and cnidarians, the apical localization of MlPar-6 induces the phosphorylation of MlPar-1, displacing this protein to basolateral cortical regions (Ohno et al., 2015; Salinas-Saavedra et al., 2015; Ragkousi et al., 2017; Salinas-Saavedra et al., 2018). Using our specific MlPar-1 antibody, we characterized the subcellular localization of the MlPar-1 protein during the early M. leidyi development (Figure 3 and all its supplements). Even though MlPar-1 appears to be localized in the cortex at the cell-contact regions of early blastomeres and gastrula stages (Figure 3D–E), this antibody signal was not clear enough to be discriminated from the cytosolic distribution, possibly due to edge effects. Nevertheless, and strikingly, MlPar-1 remains as punctate aggregations distributed uniformly in the cytosol, and in some cases, co-distributes with chromosomes during mitosis (Figure 3 and Figure 3—figure supplement 2). We did not observe asymmetric localization of MlPar-1 in the cell cortex of M. leidyi embryos at any of the stages described above for MlPar-6.

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MlPar-1 protein subcellular localization during the early development of M. leidyi.

Immunostaining against MlPar-1 protein shows that this protein remains cytoplasmic during early cleavage stages (A–D). MlPar-1 protein appears as punctate aggregations distributed uniformly in the cytosol (white arrows). Yellow arrowhead indicates the zygote nucleus in (A). 8 cell-stage (D): A single optical section from a z-stack confocal series. MlPar-1 appears to be localized in the cortex at the cell-contact regions but this antibody signal was similar to its cytosolic distribution. (E–G) Between 5 and 11 hpf, MlPar-1 protein remains as punctate aggregations distributed uniformly in the cytosol (white arrows). a’ to f’ correspond to the magnifications of the regions depicted for each stage. (E) MlPar-1 appears to be localized in the cortex at the cell-contact regions (white arrowheads) but this antibody signal was similar to its cytosolic distribution. (F) MlPar-1 protein remains cytoplasmic in ectodermal cells (Ecto; c’), endodermal (Endo; d’), and ‘mesodermal’ (‘Meso’) cells. Images are maximum projections from a z-stack confocal series. Sagittal view of an 8–9 hpf embryo corresponds to a single optical section from a z-stack confocal series. Orientation axes are depicted in the figure. Morphology is shown by DAPI and tubulin immunostainings. The animal pole is towards the top. Scale bars: 20 µm.

These results were also supported in vivo when we overexpressed the mRNA encoding for MlPar-1 fused to mCherry (MlPar-1-mCherry) into M. leidyi embryos by microinjection (Figure 3—figure supplement 3). Similar to MlPar-6-mVenus mRNA overexpression, the MlPar-1-mCherry translated protein was observed after 4 hr post injection into the uncleaved egg. Our in vivo observations on living embryos confirm the localization pattern described above by using MlPar-1 antibody at gastrula stages. MlPar-1-mCherry localizes uniformly and form aggregates in the cytosol during gastrulation (4–5 hpf; Figure 3—figure supplement 3D–E and Video 1). This localization pattern remains throughout all recorded stages until cydippid juvenile stages where MlPar-1-mCherry remains cytosolic in all cells but is highly concentrated in the tentacle apparatus and underneath the endodermal canals (24 hpf; Figure 3—figure supplement 3F–G, Figure 3—figure supplement 4, and Video 2).

Video 1
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Punctuate aggregates of MlPar-1-mCherry are highly dynamic.

2.5 min in vivo recording of a gastrula embryo at 40x.

Video 2
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Z-stack of MlPar-1-mCherry expression at 24 hpf at 40X.

MlPar-6 and MlPar-1 Proteins can localize like host proteins localize in a heterologous system

To discount the possibility that the observations recorded in vivo for both MlPar-6-mVenus and MlPar-1-mCherry proteins are caused by a low-quality mRNA or lack of structural conservation, we overexpressed each ctenophore mRNA into embryos of the cnidarian Nematostella vectensis and followed their localization by in vivo imaging (Figure 4). In N. vectensis embryos, MlPar-6-mVenus and MlPar-1-mCherry symmetrically distribute during early cleavage stages (Figure 4A and C) and both proteins localize asymmetrically only after blastula formation (Figure 4B and D). In these experiments, both MlPar-6-mVenus and MlPar-1-mCherry translated proteins display the same pattern as the previously described endogenous N. vectensis Par-6 and Par-1 proteins (Salinas-Saavedra et al., 2015). These data suggest that the protein structure of ctenophore MlPar-6 and MlPar-1 contains the necessary information to localize as other bilaterians proteins do.

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Expression of ctenophore MlPar6-mVenus and MlPar1-mCherry in embryos of the cnidarian N. vectensis.

The translated exogenous proteins display the same pattern than the previously described for endogenous N. vectensis proteins (A–D). White arrowheads indicate MlPar6-mVenus and MlPar1-mCherry cortical localization (B and D). All images are a single slice from a z-stack confocal series. (E) Graphical depiction of fluorescence intensity measurements between basal and apical cortex. The diagram at the left shows the direction of the measurements represented in this figure and in Figure 4—figure supplement 2. Median, 95% CI, and P values are depicted in the figure.

Discussion

Par protein asymmetry is established early but not maintained during M. leidyi embryogenesis

The asymmetric localization of the Par/aPKC complex has been used as an indicator of apical-basal cell polarity in a set of animals, including bilaterians (Ohno et al., 2015; Salinas-Saavedra et al., 2015; Besson et al., 2015; Yang and Mlodzik, 2015; Goldstein and Macara, 2007; Munro and Bowerman, 2009; Doerflinger et al., 2010; Chan and Nance, 2013; Lang and Munro, 2017; Mizuno et al., 2003; Kemphues et al., 1988; Etienne-Manneville and Hall, 2003; Vinot et al., 2005; Lee et al., 2007; Martindale and Hejnol, 2009; Martindale and Lee, 2013; Chalmers et al., 2005; Hayase et al., 2013) and a cnidarian (Salinas-Saavedra et al., 2015; Ragkousi et al., 2017). While in the studied bilaterians this asymmetry is established and maintained since the earliest stages of development (Munro and Bowerman, 2009; Lang and Munro, 2017; Zhu et al., 2017; Nance, 2014; Hoege and Hyman, 2013; Von Stetina and Mango, 2015), in the cnidarian N. vectensis there is no early asymmetrical localization of any of the Par components (Salinas-Saavedra et al., 2015; Ragkousi et al., 2017) and embryonic polarity is controlled by the Wnt signaling system (Kumburegama et al., 2011; Wikramanayake et al., 2003; Lee et al., 2007; Martindale and Hejnol, 2009; Martindale and Lee, 2013). In spite of these differences, once epithelial tissues form and epithelial cell-polarity is established in both bilaterian and cnidarian species, the asymmetric localization of Par proteins become highly polarized and is maintained through development. In those cases, Par-mediated apicobasal cell polarity is responsible for the maturation and maintenance of cell-cell adhesion in epithelial tissue (Ohno et al., 2015; Salinas-Saavedra et al., 2018). We have suggested that the polarizing activity of the Par system was already present in epithelial cells of the MRCA between Bilateria and Cnidaria (Salinas-Saavedra and Martindale, 2018; Salinas-Saavedra and Martindale, 2018) and could be extended to all Metazoa, where these proteins are present (including ctenophores, sponges, and placozoans Fahey and Degnan, 2010; Belahbib et al., 2018).

However, our current data suggest a different scenario for ctenophores where the Par protein polarization observed during earlier stages (characterized by the apical and cortical localization of MlPar-6; Figure 2) is not maintained when ctenophore juvenile epithelial tissues form after nine hpf. Epithelial cells of later cydippid stages do not display an asymmetric localization of MlPar-6 (Figure 2—figure supplement 6). Furthermore, the subcellular localization of MlPar-1 does not display a clear localization during any of the observed developmental stages (Figure 3 and all its supplements). Instead, punctate aggregates distribute symmetrically in the cytosol. MlPar-1 and mCherry aggregates may be consequence of the highly protein availability in the cytosol that is not captured to the cell cortex.

The components of the ctenophore MlPar/aPKC complex (MlPar-3/MlaPKC/MlPar-6 and MlCdc42) are highly conserved and contain all the domains present in other metazoans (Figure 1—figure supplements 1–2; Fahey and Degnan, 2010; Belahbib et al., 2018). Similarly, the primary structure of MlPar-1 protein (a Serine/threonine-protein kinase) is highly conserved and contains all the domains (with the same amino acid length) required for its proper functioning in other metazoans (Figure 1—figure supplement 3; Fahey and Degnan, 2010; Belahbib et al., 2018), and localizes to the lateral cortex when expressed in cnidarian embryos (Figure 4). Regardless, these proteins do not asymmetrically localize to the cortex of M. leidyi juvenile epithelium. Interestingly, the punctuate aggregates of MlPar-1-mCherry are highly dynamic and move throughout the entire cytosol (Figure 3—figure supplement 3), suggesting a potential association with cytoskeletal components (see Video 1) as MlPar-1 conserve these motifs.

Recent studies have shown that ctenophores do not have homologs for any of the Crb complex components (Belahbib et al., 2018), required for the proper stabilization of the CCC and Par/aPKC complex in other studied taxa (Ohno et al., 2015; Harris and Peifer, 2004; Tepass, 2012; Chalmers et al., 2005; Hayase et al., 2013; Whitney et al., 2016). The lack of MlPar-6 (Figure 2) polarization during later stages is totally congruent with these observations, indicating that Par proteins in ctenophores do not have the necessary interactions to stabilize apico-basal cell polarity in their cells as in other animals. In addition, ctenophore species do not have the molecular components to form SJs and lack a Scribble homolog (Belahbib et al., 2018; Ganot et al., 2015). This could explain the cytosolic localization of MlPar-1 during the observed stages (Benton and St Johnston, 2003; Iden and Collard, 2008; Humbert et al., 2015; Bilder et al., 2000; Vaccari et al., 2005), (Bonello et al., 2019).

Evolution of cell polarity and epithelial structure in metazoa

Given the genomic conservation of cell-polarity components in the Bilateria and Cnidaria, we propose to classify their epithelium as ‘Par-dependent’ to include its mechanistic regulatory properties. That is, the structural properties of a ‘Par-dependent’epithelium are the result of conserved interactions between subcellular pathways that polarize epithelial cells. Thus, when we seek to understand the origins of the epithelial nature of one particular tissue, we are trying to understand the synapomorphies (shared derived characters) of the mechanisms underlying the origin of that particular tissue. Under this definition, a ‘Par-dependent epithelium’ may have a single origin in Metazoa, but, different mechanisms might have co-opted to generate similar epithelial morphologies (Figure 4—figure supplement 1). Ctenophore epithelia, along with other recent works in N. vectensis endomesoderm (Salinas-Saavedra et al., 2015; Salinas-Saavedra et al., 2018) and Drosophila midgut (Chen et al., 2018), suggest this possibility. In all these cases, epithelial cells are highly polarized along the apical-basal axis, but this polarization does not depend on Par proteins. Therefore, these cells are not able to organize a ‘Par-dependent epithelium’ (mechanistic definition) but still polarized epithelial morphologies.

Genomic studies also suggest that ctenophore species lack the molecular interactions necessaries to form the apical cell polarity and junctions observed in Cnidaria + Bilateria. Intriguingly, ctenophore genomes do not have the Wnt signaling pathway components (Ryan et al., 2013; Moroz et al., 2014; Pang et al., 2010) that control the activity of Par proteins in bilaterian and cnidarian embryos (components that are also present in poriferan and placozoan genomes Belahbib et al., 2018). For example, in bilaterians the Wnt/PCP signaling pathway antagonizes the action of the Par/aPKC complex (Cha et al., 2011; Besson et al., 2015; Aigouy and Le Bivic, 2016; Humbert et al., 2015; Humbert et al., 2006; Seifert and Mlodzik, 2007), so this may explain the lack of polarization in ctenophore tissue. Furthermore, ctenophore species do not have the full set of cell-cell adhesion proteins (Belahbib et al., 2018; Ryan et al., 2013; Ganot et al., 2015) as we know them in other metazoans, including Placozoans and Poriferans (Magie and Martindale, 2008; Belahbib et al., 2018). The cadherin of ctenophores does not have the cytoplasmic domains required to bind any of the catenins of the CCC (e.g. p120, alpha- and ß-catenin) (Belahbib et al., 2018). This implies that neither the actin nor microtubule cytoskeleton can be linked to ctenophore cadherin through the CCC, as seen essential in other metazoans to stabilize pre-existent Par proteins polarity. This suggests that there are additional mechanisms that integrate the cytoskeleton of ctenophore cells with their cell-cell adhesion system.

In conclusion, regardless the phylogenetic position of the Ctenophora, the conservation of an organized ‘Par-dependent epithelium’ cannot be extended to all Eumetazoa. Ctenophore cells do not have other essential components to organize the polarizing function of the Par system as in other studied metazoans. Despite the high structural conservation of Par proteins across Metazoa, we have shown that ctenophore cells do not deploy and/or stabilize the asymmetrical localization of Par-6 and Par-1 proteins. Thus, ctenophore tissues organize their epithelium in a different way than the classical definition seen in bilaterians. In agreement with genomic studies, our results question what molecular properties defined the ancestral roots of a metazoan epithelium, and whether similar epithelial morphologies (e.g., epidermis and mesoderm) could be developed by independent or modifications of existing cellular and molecular interactions (including cell adhesion systems). Unless the lack of Par protein localization in M. leidyi is a secondary loss, the absence of these pathways in ctenophores implies that a new set of interactions emerged at least in the Cnidaria+Bilateria ancestor (Figure 4—figure supplement 1), and that, could have regulated the way by which the Par system polarizes embryonic and epithelial cells. While bioinformatic studies are critical to understand the molecular composition, we need further research to understand how these molecules actually interact with one another to organize cellular behavior (e.g., integrin-collagen, basal-apical interactions) in a broader phylogenetical sample, including Porifera and Placozoa.

Materials and methods

Key resources table
Reagent type
(species) or
resource		DesignationSource or
reference		IdentifiersAdditional information
AntibodyMouse Anti-alpha-Tubulin Monoclonal Antibody, Unconjugated, Clone DM1ASigma-AldrichT9026; RRID:AB_477593(1:500)
Antibodyanti-MlPar-6 custom peptide antibody produced in rabbitBethyl labs; This studyStored at MQ Martindale's lab; (1:100)
Antibodyanti-MlPar-1 custom peptide antibody produced in rabbitBethyl labs; This studyStored at MQ Martindale's lab; (1:100)
AntibodyGoat anti-Mouse
IgG Secondary Antibody, Alexa Fluor 568		Thermo Fisher ScientificA-11004; RRID:AB_2534072(1:250)
AntibodyGoat anti-Rabbit IgG Secondary Antibody, Alexa Fluor 647Thermo Fisher ScientificA-21245; RRID:AB_2535813(1:250)
OtherDAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)Thermo Fisher ScientificD1306; RRID:AB_2629482(0.1 µg/µl)
Chemical compound, drugDextran, Alexa Fluor 488; 10,000 MW, Anionic, FixableThermo Fisher ScientificD22910
Chemical compound, drugDextran, Alexa Fluor 555; 10,000 MW, Anionic, FixableThermo Fisher ScientificD34679
Chemical compound, drugDextran, Alexa Fluor 647; 10,000 MW, Anionic, FixableThermo Fisher ScientificD22914
Chemical compound, drugDextran, Cascade Blue, 10,000 MW, Anionic, Lysine FixableThermo Fisher ScientificD1976
Sequence-based reagentMlpar-6: F-GTACTGTGCTGTGTGTTTGGA; R- GTACTGTGCTGTGTGTTTGGAMnemiopsis Genome Project - NIH-NHGRIMLRB351777
Sequence-based reagentMlpar-1: F- ATGTCAAATTCTCAACACCAC; R- CAGTCTTAATTCATTAGCTATGTTAMnemiopsis Genome Project - NIH-NHGRIMLRB182569
Recombinant DNA reagentpSPE3-mVenusRoure et al., 2007Gateway vector
Recombinant DNA reagentpSPE3-mCherryRoure et al., 2007Gateway vector
Software, algorithmFiji (ImageJ)NIHhttp://fiji.sc
Software, algorithmImaris 7.6.4Bitplane Inc

Culture and spawning of M. leidyi

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Spawning, gamete preparation, fertilization and embryo culturing of M. leidyi at the Whitney Laboratory for Marine Bioscience of the University of Florida (USA)embryos was performed as previously described (Salinas-Saavedra and Martindale, 2018).

Western blot

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Western blots were carried out as described (Salinas-Saavedra et al., 2015; Salinas-Saavedra et al., 2018) using adult epithelial tissue lysates dissected by hand in order to discard larger amount of mesoglea. Antibody concentrations for Western blot were 1:1000 for all antibodies tested.

Immunohistochemistry

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All immunohistochemistry experiments were carried out using the previous protocol for M. leidyi (Salinas-Saavedra and Martindale, 2018). The primary antibodies and concentrations used were: mouse anti-alpha tubulin (1:500; Sigma-Aldrich, Inc Cat.# T9026. RRID:AB_477593). Secondary antibodies are listed in the Key Resources table. Rabbit anti-MlPar-6, and rabbit anti-MlPar-1 antibodies were custom made high affinity-purified peptide antibodies that commercially generated by Bethyl labs, Inc (Montgomery, TX, USA). Affinity-purified M. leidyi anti-Par-6 (anti-MlPar-6) and anti-Par-1 (anti-MlPar-1) peptide antibodies were raised against a selected amino acid region of the MlPar-6 protein (MTYPDDSNGGSGR) and MlPar-1 protein (KDIAVNIANELRL), respectively. Blast searches against the M. leidyi genome sequences showed that the amino acid sequences were not present in any predicted M. leidyi proteins other than the expected protein. Both antibodies are specific to M. leidyi proteins (Figure 2—figure supplement 2) and were diluted 1:100.

mRNA microinjections

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The coding region for each gene of interest was PCR-amplified using cDNA from M. leidyi embryos and cloned into pSPE3-mVenus or pSPE3-mCherry using the Gateway system (Roure et al., 2007). To confirm the presence of the transcripts during M. leidyi development, we cloned each gene at 2 hpf and 48 hpf. N. vectensis eggs were injected directly after fertilization as previously described (Salinas-Saavedra et al., 2015; DuBuc et al., 2014; Layden et al., 2013) with the mRNA encoding one or more proteins fused in frame with reporter fluorescent protein (N-terminal tag) using an optimized final concentration of 300 ng/µl for each gene. Fluorescent dextran was also co-injected to visualize the embryos. Live embryos were kept at room temperature and visualized after the mRNA of the FP was translated into protein (4–5 hr). Live embryos were mounted in 1x sea water for visualization. Images were documented at different stages. We injected and recorded at least 20 embryos for each injected protein and confocal imaged each specimen at different stages for detailed analysis of phenotypes in vivo. We repeated each experiment at least five times obtaining similar results for each case. The fluorescent dextran and primers for the cloned genes are listed in Key resources table.

Imaging of M. leidyi embryos

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Images of live and fixed embryos were taken using a confocal Zeiss LSM 710 microscope using a Zeiss C-Apochromat 40x water immersion objective (N.A. 1.20). Pinhole settings varied between 1.2–1.4 A.U. according to the experiment. The same settings were used for each individual experiment to compare control and experimental conditions. Z-stack images were processed using Imaris 7.6.4 (Bitplane Inc) software for three-dimensional reconstructions and FIJI for single slice and videos. Final figures were assembled using Adobe Illustrator and Adobe Photoshop.

Par proteins display a general cytosolic localization when their polarizing activity is inactive. This signal was diminished by modifying contrast and brightness of the images in order to enlighten their cortical localization (active state in cell-polarity and stronger antibody signal) as it has shown in other organisms. All RAW images are available upon request.

Fluorescent intensity measurements and statistical analyses

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Images of fixed embryos were measured using FIJI plot profile tool using the RAW source data. Fluorescent intensity was measured along the animal-vegetal axis for 1 and 2 cell stages and along the apico-basal axis for the other later stages. The data obtained were then normalized by the maximum value of each X and Y axes. X axis corresponds to the distance from basal (0) to apical (1) cortex. Y axis corresponds to fluorescence intensity. The normalized data were plotted and the numerical values can be found in figure supplement-data source files. For later stages than 8 cells, we took measurements of two cells located in perpendicular axes of the embryo where the apicobasal axis was clearly detectable. These measurements correspond to cells going through interphase and metaphase. Statistical analyses were executed using GraphPad prism software. To do this, we compared the 10% most basal positions with the 10% most apical positions for each stage. We plotted this data and differences were assessed by comparing medians using Mann-Whitney U test.

Similarly, fluorescent intensity during cell cycle (Figure 2—figure supplement 11) was measured along the apical cortex. The data obtained were then normalized by the maximum value of each X and Y axes. X axis corresponds to the arbitrary distance (0 to 1) along the apical cortex where the middle point corresponds to the cell-cell contact region or cleavage furrow. Y axis corresponds to fluorescence intensity. The normalized data were plotted and the numerical values can be found in Figure 2—figure supplement 11—source data 1.

Data availability

Genomic and Sequencing data can be found in the Mnemiopsis Genome Project (NIH-NHGRI) webpage (http://kona.nhgri.nih.gov/mnemiopsis/). We used the genome and prediction models to search the sequences of Par6 (https://kona.nhgri.nih.gov/mnemiopsis/jbrowse/data.cgi?type=unfiltered2.2&gene=MLRB351777) and Par1 (https://kona.nhgri.nih.gov/mnemiopsis/jbrowse/data.cgi?type=unfiltered2.2&gene=MLRB182569) using the blast tool. All data generated or analyzed during this study are included in the manuscript and supporting files.

The following data sets were generated
    1. Salinas-Saavedra M
    2. Martindale MQ
    (2020) BioStudies
    ID S-BSST502. Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the Metazoa.
    https://www.ebi.ac.uk/biostudies/studies/S-BSST502

References

    1. Johansson A
    2. Driessens M
    3. Aspenström P
    (2000)
    The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the rho GTPases Cdc42 and Rac1
    Journal of Cell Science 113:3267–3275.
    1. Macara IG
    (2004) Parsing the polarity code
    Nature Reviews Molecular Cell Biology 5:220–231.
    https://doi.org/10.1038/nrm1332
  58. Book
    1. Ohno S
    2. Goulas S
    3. Hirose T
    (2015) The PAR3-aPKC-PAR6 complex. in cell polarity 1
    In: Ebnet K, editors. Biological Role and Basic Mechanisms. Springer. pp. 3–23.
    https://doi.org/10.1007/978-3-319-14463-4_1

Article and author information

Author details

  1. Miguel Salinas-Saavedra

    The Whitney Laboratory for Marine Bioscience, and the Department of Biology, University of Florida, St. Augustine, United States
    Present address
    Centre for Chromosome Biology, Bioscience Building, National University of Ireland Galway, Galway, Ireland
    Contribution
    Conceptualization, Resources, Validation, Investigation, Visualization, Methodology, Writing - original draft, Project administration, Writing - review and editing
    For correspondence
    miguel.salinas-saavedra@nuigalway.ie
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1598-9881

  2. Mark Q Martindale

    The Whitney Laboratory for Marine Bioscience, and the Department of Biology, University of Florida, St. Augustine, United States
    Contribution
    Conceptualization, Resources, Supervision, Funding acquisition, Writing - original draft, Project administration, Writing - review and editing
    For correspondence
    mqmartin@whitney.ufl.edu
    Competing interests
    No competing interests declared

Funding

National Science Foundation (NSF IOS-1755364)

  • Mark Q Martindale

National Aeronautics and Space Administration (NASA 16-EXO16_2-0041)

  • Mark Q Martindale

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank J Torres-Paz, CE Schnitzler, U Frank, and J Ryan for technical assistance and helpful comments.

Version history

  1. Received: January 6, 2020
  2. Accepted: July 23, 2020
  3. Accepted Manuscript published: July 27, 2020 (version 1)
  4. Accepted Manuscript updated: July 30, 2020 (version 2)
  5. Version of Record published: August 20, 2020 (version 3)

Copyright

© 2020, Salinas-Saavedra and Martindale

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. Miguel Salinas-Saavedra
  2. Mark Q Martindale
(2020)
Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa
eLife 9:e54927.
https://doi.org/10.7554/eLife.54927

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