A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).
SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.
Rachida Yakoubi, Astrid Rollenhagen ... Joachim HR Lübke
Neocortical synapses in layer 4 of the human temporal lobe neocortex were quantitatively characterized, at the subcellular level, using high-end, high-resolution electron microscopy and 3D-volume reconstructions.
Structural analysis of an AKAP18-protein kinase A complex reveals that regions of intrinsic disorder impact the phosphorylation of preferred substrates.
Benedikt Staffler, Manuel Berning ... Moritz Helmstaedter
The detection of chemical synapses in 3-dimensional electron microscopy data has been automated such that synapses in large-scale connectivity maps of the cerebral cortex, connectomes, can be charted without the need for human interaction.
The nuclear export receptor Crm1 cooperatively binds its HIV Rev-RRE cargo as a dimer using a species-specific interface that supports viral replication by enhancing nuclear export of HIV RNA.
Bronwyn A Lucas, Benjamin A Himes, Nikolaus Grigorieff
2D template matching enables a streamlined single-particle cryogenic electron microscopy workflow that can be used to discover new high-resolution structural features in molecules and complexes, such as bound ligands and drugs.
Manuel Alonso Y Adell, Simona M Migliano ... David Teis
Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.
Fengwei Zheng, Roxana Georgescu ... Michael E O'Donnell
Cryo-EM shows a 5’ DNA site on the RFC clamp loader, in addition to the 3’ site, and biochemical study shows the 5’ site facilitates PCNA loading onto gaps, consistent with genetics indicating the 5’ DNA site facilitates gap repair.