2,461 results found
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

    Tin Ki Tsang, Eric A Bushong ... Mark H Ellisman
    A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).
    1. Cell Biology

    A robust method for particulate detection of a genetic tag for 3D electron microscopy

    James Rae, Charles Ferguson ... Robert G Parton
    Development of a new system for gold particle detection of a genetic tag for electron microscopy.
    1. Structural Biology and Molecular Biophysics

    A modular platform for automated cryo-FIB workflows

    Sven Klumpe, Herman KH Fung ... Julia Mahamid
    SerialFIB is an adaptable open-source software designed for streamlining, advanced development and automation of cryo-focused ion beam-based cellular preparations for in situ cryo-electron tomography.
    1. Neuroscience

    Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex

    Rachida Yakoubi, Astrid Rollenhagen ... Joachim HR Lübke
    Neocortical synapses in layer 4 of the human temporal lobe neocortex were quantitatively characterized, at the subcellular level, using high-end, high-resolution electron microscopy and 3D-volume reconstructions.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Intrinsic disorder within an AKAP-protein kinase A complex guides local substrate phosphorylation

    F Donelson Smith, Steve L Reichow ... Tamir Gonen
    Structural analysis of an AKAP18-protein kinase A complex reveals that regions of intrinsic disorder impact the phosphorylation of preferred substrates.
    1. Neuroscience

    SynEM, automated synapse detection for connectomics

    Benedikt Staffler, Manuel Berning ... Moritz Helmstaedter
    The detection of chemical synapses in 3-dimensional electron microscopy data has been automated such that synapses in large-scale connectivity maps of the cerebral cortex, connectomes, can be charted without the need for human interaction.
    1. Structural Biology and Molecular Biophysics
    2. Microbiology and Infectious Disease

    The export receptor Crm1 forms a dimer to promote nuclear export of HIV RNA

    David S Booth, Yifan Cheng, Alan D Frankel
    The nuclear export receptor Crm1 cooperatively binds its HIV Rev-RRE cargo as a dimer using a species-specific interface that supports viral replication by enhancing nuclear export of HIV RNA.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias

    Bronwyn A Lucas, Benjamin A Himes, Nikolaus Grigorieff
    2D template matching enables a streamlined single-particle cryogenic electron microscopy workflow that can be used to discover new high-resolution structural features in molecules and complexes, such as bound ligands and drugs.
    1. Cell Biology

    Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding

    Manuel Alonso Y Adell, Simona M Migliano ... David Teis
    Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.
    1. Biochemistry and Chemical Biology

    Cryo-EM structures reveal that RFC recognizes both the 3′- and 5′-DNA ends to load PCNA onto gaps for DNA repair

    Fengwei Zheng, Roxana Georgescu ... Michael E O'Donnell
    Cryo-EM shows a 5’ DNA site on the RFC clamp loader, in addition to the 3’ site, and biochemical study shows the 5’ site facilitates PCNA loading onto gaps, consistent with genetics indicating the 5’ DNA site facilitates gap repair.

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